5-ALA benzyl ester hydrochloride (Synonyms: Benzyl-ALA hydrochloride)

Name: 5-ALA benzyl ester hydrochloride
Brand: MedChemExpress
SKU: HY-133100
Rating: 4.5 (1 reviews)

Cat. No.: HY-133100 Purity: 99.44%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

5-ALA benzyl ester hydrochloride (Benzyl-ALA hydrochloride) is a protoporphyrin precursor used as a photodetection agent. 5-ALA benzyl ester hydrochloride induces protoporphyrin IX (PPIX) accumulation in colon carcinoma cell lines.

For research use only. We do not sell to patients.

5-ALA benzyl ester hydrochloride Chemical Structure

CAS No. : 163271-32-7

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 72	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 72	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 41	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 65	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 220	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 370	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

In Vitro

5-ALA benzyl ester hydrochloride (Benzyl-ALA hydrochloride) (0.6-0.12 mM; 3 h) induces protoporphyrin IX (PPIX) accumulation in colon carcinoma cell lines SW480^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Reducing the dose of 5-ALA benzyl ester hydrochloride (31 mg/kg; i.v. ; 1 hours) results in a dose-dependent reduction in tumour porphyrin levels^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female CBA/Gy mouse (CaNT tumour model)^[3]
Dosage:	31 mg/kg
Administration:	i.v. ; 1 hours
Result:	Reducing the dose of 5-ALA benzyl ester hydrochloride resulted in a dose-dependent reduction in the concentration of porphyrins accumulating in the CaNT tumour.

Molecular Weight

257.71

Formula

C₁₂H₁₆ClNO₃

CAS No.

163271-32-7

Appearance

Solid

Color

White to off-white

SMILES

O=C(OCC1=CC=CC=C1)CCC(CN)=O.[H]Cl

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (970.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (388.03 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8803 mL	19.4017 mL	38.8033 mL
5 mM	0.7761 mL	3.8803 mL	7.7607 mL
10 mM	0.3880 mL	1.9402 mL	3.8803 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (8.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (8.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (8.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (388.03 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.44%

Select Batch:

Data Sheet (271 KB) SDS (252 KB)

COA (244 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Hirschberg H, et al. ALA- and ALA-ester-mediated photodynamic therapy of human glioma spheroids. J Neurooncol. 2002 Mar;57(1):1-7. [Content Brief]

[2]. Brunner H, et al. The effects of 5-aminolevulinic acid esters on protoporphyrin IX production in human adenocarcinoma cell lines. Photochem Photobiol. 2001 Nov;74(5):721-5. [Content Brief]

[3]. Tunstall RG, et al. Porphyrin accumulation induced by 5-aminolaevulinic acid esters in tumour cells growing in vitro and in vivo. Br J Cancer. 2002 Jul 15;87(2):246-50. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.8803 mL	19.4017 mL	38.8033 mL	97.0083 mL
	5 mM	0.7761 mL	3.8803 mL	7.7607 mL	19.4017 mL
	10 mM	0.3880 mL	1.9402 mL	3.8803 mL	9.7008 mL
	15 mM	0.2587 mL	1.2934 mL	2.5869 mL	6.4672 mL
	20 mM	0.1940 mL	0.9701 mL	1.9402 mL	4.8504 mL
	25 mM	0.1552 mL	0.7761 mL	1.5521 mL	3.8803 mL
	30 mM	0.1293 mL	0.6467 mL	1.2934 mL	3.2336 mL
	40 mM	0.0970 mL	0.4850 mL	0.9701 mL	2.4252 mL
	50 mM	0.0776 mL	0.3880 mL	0.7761 mL	1.9402 mL
	60 mM	0.0647 mL	0.3234 mL	0.6467 mL	1.6168 mL
	80 mM	0.0485 mL	0.2425 mL	0.4850 mL	1.2126 mL
	100 mM	0.0388 mL	0.1940 mL	0.3880 mL	0.9701 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

5-ALA benzyl ester hydrochloride Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-ALA benzyl ester163271-32-7Benzyl-ALAEndogenous MetaboliteprotoporphyrinphotodetectionaccumulationcoloncarcinomaSW480Inhibitorinhibitorinhibit

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5-ALA benzyl ester hydrochloride (Synonyms: Benzyl-ALA hydrochloride)

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5-ALA benzyl ester hydrochloride Related Antibodies

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Complete Stock Solution Preparation Table

5-ALA benzyl ester hydrochloride Related Classifications

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