1. Neuronal Signaling Autophagy
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  3. Autocamtide-2-related inhibitory peptide TFA

Autocamtide-2-related inhibitory peptide TFA 

Cat. No.: HY-P0214A Purity: 95.24%
COA Handling Instructions

Autocamtide-2-related inhibitory peptide (TFA) is a highly specific and potent inhibitor of CaMKII with an IC50 of 40 nM.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Autocamtide-2-related inhibitory peptide TFA Chemical Structure

Autocamtide-2-related inhibitory peptide TFA Chemical Structure

Size Price Stock Quantity
1 mg USD 120 In-stock
5 mg USD 290 In-stock
10 mg USD 435 In-stock
50 mg   Get quote  
100 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Autocamtide-2-related inhibitory peptide TFA:

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Autocamtide-2-related inhibitory peptide TFA

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Autocamtide-2-related inhibitory peptide (TFA) is a highly specific and potent inhibitor of CaMKII with an IC50 of 40 nM.

IC50 & Target

CaMK II

 

In Vitro

It is found that Autocamtide-2-related inhibitory peptide (TFA) is a potent inhibitor of CaM-kinase II with an IC50 of 40 nM, which is active in the presence or absence of Ca2+/calmodulin. It is 50 and 500 times more potent than CaMK-(281-302Ala286) and KN-93, respectively, under the assay condition used[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1611.76

Formula

C66H126N22O21F3

Appearance

Solid

Color

White to off-white

Sequence

Lys-Lys-Ala-Lru-Arg-Arg-Gln-Glu-Ala-Val-Asp-Ala-Leu

Sequence Shortening

KKALRRQEAVDAL

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 110 mg/mL (68.25 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.6204 mL 3.1022 mL 6.2044 mL
5 mM 0.1241 mL 0.6204 mL 1.2409 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (31.02 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.6204 mL 3.1022 mL 6.2044 mL 15.5110 mL
5 mM 0.1241 mL 0.6204 mL 1.2409 mL 3.1022 mL
10 mM 0.0620 mL 0.3102 mL 0.6204 mL 1.5511 mL
15 mM 0.0414 mL 0.2068 mL 0.4136 mL 1.0341 mL
20 mM 0.0310 mL 0.1551 mL 0.3102 mL 0.7755 mL
25 mM 0.0248 mL 0.1241 mL 0.2482 mL 0.6204 mL
30 mM 0.0207 mL 0.1034 mL 0.2068 mL 0.5170 mL
40 mM 0.0155 mL 0.0776 mL 0.1551 mL 0.3878 mL
50 mM 0.0124 mL 0.0620 mL 0.1241 mL 0.3102 mL
60 mM 0.0103 mL 0.0517 mL 0.1034 mL 0.2585 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Autocamtide-2-related inhibitory peptide TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Autocamtide-2-related inhibitory peptide TFA
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HY-P0214A
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