1. Cell Cycle/DNA Damage
    Epigenetics
  2. HDAC
  3. ITSA-1

ITSA-1 

Cat. No.: HY-100508 Purity: >98.0%
Handling Instructions

ITSA-1 is an activator of histone deacetylase (HDAC), and counteract trichostatin A (TSA)-induced cell cycle arrest, histone acetylation, and transcriptional activation.

For research use only. We do not sell to patients.

ITSA-1 Chemical Structure

ITSA-1 Chemical Structure

CAS No. : 200626-61-5

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
10 mg USD 60 In-stock
Estimated Time of Arrival: December 31
25 mg USD 84 In-stock
Estimated Time of Arrival: December 31
50 mg USD 144 In-stock
Estimated Time of Arrival: December 31
100 mg USD 240 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 3 publication(s) in Google Scholar

Top Publications Citing Use of Products

    ITSA-1 purchased from MCE. Usage Cited in: BMC Cancer. 2019 Mar 22;19(1):262.

    Western blot is used to examine the protein levels of cell proliferation (PCNA), cell cycle (cyclinD1, CDK2 and p16) and EMT-associated genes (E-cadherin and Vimentin) in the treatment of TSA or TSA plus ITSA-1.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    ITSA-1 is an activator of histone deacetylase (HDAC), and counteract trichostatin A (TSA)-induced cell cycle arrest, histone acetylation, and transcriptional activation[1].

    IC50 & Target[1]

    HDAC

     

    In Vitro

    ITSA1 (50 μM; A549 cells) treatment serves to revert the TSA-arrested population to a normal cell cycle distribution. ITSA1 is also able to effect cell cycle rescue over longer duration[1].
    ITSA1 (50 μM; 5 hours; A549 cells) treatment reduces the number of apoptosis in TSA-treated cells[1].
    ITSA1 (50 μM; 2 hours; A549 and murine ES cells cells) treatment suppresses TSA-induced histone acetylation. Importantly, suppression of acetylation levels is only observable when ITSA1 is added concurrent with or post TSA treatment[1].
    ITSA1 (50 μM; 30 minutes; murine ES cells cells) suppresses TSA-activated transcription in murine ES cells[1].

    Cell Cycle Analysis[1]

    Cell Line: Murine ES cells
    Concentration: 50 μM
    Incubation Time:
    Result: Served to revert the TSA-arrested population to a normal cell cycle distribution.

    Apoptosis Analysis[1]

    Cell Line: A549 cells
    Concentration: 50 μM
    Incubation Time: 5 hours
    Result: Reduced the number of apoptosis.

    Western Blot Analysis[1]

    Cell Line: A549 and murine ES cells
    Concentration: 50 μM
    Incubation Time: 2 hours
    Result: Reduced histone acetylation to the baseline level.

    RT-PCR[1]

    Cell Line: Murine ES cells
    Concentration: 50 μM
    Incubation Time: 30 minutes
    Result: Suppressed TSA-activated transcription.
    In Vivo

    ITSA-1 (0.5 mg/kg; intraperitoneal injection; 3 times/week; for 8 weeks; CBS+/− mice) treatment balances deacetylation activity and suppresses IL-6 and TNF-α expression and thereby attenuated histone acetylationdependent infammatory signaling[2].

    Animal Model: CBS+/− mice[2]
    Dosage: 0.5 mg/kg
    Administration: Intraperitoneal injection; 3 times/week; for 8 weeks
    Result: Balanced deacetylation activity and suppressed IL-6 and TNF-α expression.
    Molecular Weight

    292.12

    Formula

    C₁₃H₇Cl₂N₃O

    CAS No.

    200626-61-5

    SMILES

    O=C(N1N=NC2=CC=CC=C21)C3=CC=C(Cl)C=C3Cl

    Shipping

    Room temperature in continental US; may vary elsewhere

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 32 mg/mL (109.54 mM)

    H2O : < 0.1 mg/mL (insoluble)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.4233 mL 17.1163 mL 34.2325 mL
    5 mM 0.6847 mL 3.4233 mL 6.8465 mL
    10 mM 0.3423 mL 1.7116 mL 3.4233 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (8.56 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 2.5 mg/mL (8.56 mM); Suspended solution; Need ultrasonic

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (8.56 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.
    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product name

     

    Salutation

    Applicant name *

     

    Email address *

    Phone number *

     

    Organization name *

    Country or Region *

     

    Requested quantity *

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    ITSA-1
    Cat. No.:
    HY-100508
    Quantity: