2. Protein Tyrosine Kinase/RTK Immunology/Inflammation GPCR/G Protein JAK/STAT Signaling Stem Cell/Wnt
  3. VEGFR CCR STAT
  4. Medrysone

Medrysone  (Synonyms: HMS; 6α-Methyl-11β-hydroxyprogesterone)

Cat. No.: HY-B1076 Purity: 99.79%
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Medrysone (HMS; 6α-Methyl-11β-hydroxyprogesterone) is a STAT6 modulator and M2 macrophage polarization inducer. Medrysone enhances IL-4-triggered STAT6 activation, upregulates the expression of M2 markers, and promotes the secretion of VEGF and CCL2. Medrysone also enhances the pro-migratory activity of M2-like macrophages toward endothelial cells. By regulating macrophage polarization and related repair pathways, Medrysone significantly promotes corneal wound repair in a rat mechanical injury model. Medrysone can be used for research related to corneal injury.

For research use only. We do not sell to patients.

Medrysone

Medrysone Chemical Structure

CAS No. : 2668-66-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
Solid
25 mg In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Medrysone:

Medrysone Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Medrysone

View All VEGFR Isoform Specific Products:

View All Isoforms
VEGFR1/Flt-1 VEGFR2/KDR/Flk-1 VEGFR3/Flt-4

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CCR1 CCR2 CCR3 CCR4 CCR5 CCR6 CCR7 CCR8 CCR9

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STAT3 STAT5 STAT6 STAT1

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Medrysone (HMS; 6α-Methyl-11β-hydroxyprogesterone) is a STAT6 modulator and M2 macrophage polarization inducer. Medrysone enhances IL-4-triggered STAT6 activation, upregulates the expression of M2 markers, and promotes the secretion of VEGF and CCL2. Medrysone also enhances the pro-migratory activity of M2-like macrophages toward endothelial cells. By regulating macrophage polarization and related repair pathways, Medrysone significantly promotes corneal wound repair in a rat mechanical injury model. Medrysone can be used for research related to corneal injury[1].

IC50 & Target

STAT6

 

CCR2

 

In Vitro

Medrysone (5 μM; 24 h) promotes M2 polarization of RAW264.7 and BMDM cells via a STAT6-dependent pathway, increases the proportion of CD206-positive cells, and exerts enhanced effects when used in combination with IL-4; whereas silencing STAT6 abolishes the increase in CD206-positive cells[1].
When used in combination with IL-4, Medrysone (5 μM; 24 h) upregulates the mRNA expression levels of M2 marker genes (Mrc1, IL10, Fizz1, Arg1, CD11b) in RAW264.7 cells, with the levels being higher than those observed with either agent alone[1].
When used in combination with IL-4, Medrysone (5 μM; 24 h) significantly promotes the secretion of VEGF and CCL2 by RAW264.7 cells and increases the protein level of p-STAT6[1].
Medrysone (5 μM; 24 h) enhances the pro-migratory and pro-wound healing activities of RAW264.7 cell-conditioned medium on C166 vascular endothelial cells, with the strongest effect observed when combined with IL-4[1].
Medrysone (100 nM; 21 d) increases ALP activity and supports the long-term adhesion of human mesenchymal stem cells (hMSCs) on TCPS for up to 21 days, but it induces significantly less mineralized matrix deposition compared to osteogenic complete medium supplemented with dexamethasone[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Medrysone Related Antibodies

Western Blot Analysis[1]

Cell Line: RAW264.7 murine macrophage cells
Concentration: 5 μM
Incubation Time: 24 h
Result: Increased p-STAT6 levels relative to control when used alone.
Further enhanced p-STAT6 levels to a relative value of 1.44 (short exposure) when combined with IL-4, which is higher than the 0.75 relative value observed with IL-4 alone.

Cell Differentiation Assay[1]

Cell Line: C166 murine vascular endothelial cells, RAW264.7 murine macrophage cells
Concentration: 5 μM
Incubation Time: 24 h (treatment of RAW264.7 cells)
Result: Increased C166 cell migration to a fold change of approximately 0.9 when using conditioned medium from Medrysone-treated RAW264.7 cells.
Increased C166 cell migration to a fold change of approximately 2.3 when using conditioned medium from RAW264.7 cells treated with both Medrysone and IL-4, which is higher than the fold change of approximately 1.8 observed with conditioned medium from IL-4-treated cells.\n
Enhanced C166 cell wound closure when using conditioned medium from Medrysone-treated RAW264.7 cells.
Induced the strongest wound closure when using conditioned medium from RAW264.7 cells treated with both Medrysone and IL-4.

Cell Differentiation Assay[2]

Cell Line: human mesenchymal stem cells (hMSCs)
Concentration: 1 nM; 10 nM; 100 nM
Incubation Time: up to 21 days
Result: Supported cell proliferation comparable to cells grown in complete osteogenic media (OG+) over 21 days at 100 nM, with cells remaining attached to TCPS throughout the study.
Increased ALP activity slightly relative to OG+ at 14 days, and by ~25% relative to OG+ at 21 days at 100 nM.
Elevated relative CBFA1 expression slightly above day 0 levels at 14, 18, and 21 days, but lower than OG+ levels at 18 days at 100 nM.
Increased relative osteocalcin expression over time, with levels slightly elevated above day 0 but lower than OG+ at 21 days at 100 nM.
Resulted in significantly less mineralized matrix deposition relative to OG+, with only minimal Alizarin Red staining observed after 21 days at 100 nM.
In Vivo

Medrysone (5 μM; topical; every 6 hours; 48 hours) significantly promotes corneal injury repair in rats[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: male, 6-8 weeks old[1]
Dosage: 5 μM
Administration: topical; every 6 hours; 48 hours
Result: Significantly reduced the percentage of fluorescein-positive corneal area at 48 hours.
Significantly upregulated mRNA expression of M2 macrophage markers Mrc1 (p < 0.01), Fizz1 (p < 0.01), Arg1 (p < 0.05), and CD11b (p < 0.05).
Clinical Trial
Molecular Weight

344.49

Formula

C22H32O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC([C@H]1CC[C@@]2([H])[C@]3([H])C[C@H](C)C4=CC(CC[C@]4(C)[C@@]3([H])[C@@H](O)C[C@]12C)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (145.14 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : ≥ 25 mg/mL (72.57 mM)

H2O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9028 mL 14.5142 mL 29.0284 mL
5 mM 0.5806 mL 2.9028 mL 5.8057 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.26 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (7.26 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.79%

SDS (393 KB)

COA (255 KB) HNMR (252 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 2.9028 mL 14.5142 mL 29.0284 mL 72.5710 mL
5 mM 0.5806 mL 2.9028 mL 5.8057 mL 14.5142 mL
10 mM 0.2903 mL 1.4514 mL 2.9028 mL 7.2571 mL
15 mM 0.1935 mL 0.9676 mL 1.9352 mL 4.8381 mL
20 mM 0.1451 mL 0.7257 mL 1.4514 mL 3.6286 mL
25 mM 0.1161 mL 0.5806 mL 1.1611 mL 2.9028 mL
30 mM 0.0968 mL 0.4838 mL 0.9676 mL 2.4190 mL
40 mM 0.0726 mL 0.3629 mL 0.7257 mL 1.8143 mL
50 mM 0.0581 mL 0.2903 mL 0.5806 mL 1.4514 mL
60 mM 0.0484 mL 0.2419 mL 0.4838 mL 1.2095 mL
DMSO 80 mM 0.0363 mL 0.1814 mL 0.3629 mL 0.9071 mL
100 mM 0.0290 mL 0.1451 mL 0.2903 mL 0.7257 mL
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Medrysone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Medrysone2668-66-8HMS 6α-Methyl-11β-hydroxyprogesteroneVEGFRCCRSTATVascular endothelial growth factor receptorCC chemokine receptorC166 vascular endothelial cellsBMDMsSTAT6VEGFcorneal injuryRAW264.7 cellsIL-4M2 macrophage polarizationCCL2human mesenchymal stem cellsInhibitorinhibitorinhibit

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