1. Membrane Transporter/Ion Channel
    Neuronal Signaling
  2. TRP Channel
  3. RN-1734

RN-1734 

Cat. No.: HY-19975 Purity: 99.01%
Handling Instructions

RN-1734 is selective antagonist of the TRPV4 channel, completely antagonizes 4αPDD-mediated activation of TRPV4 with comparable, low micromolar IC50s for all three species (hTRPV4: 2.3 μM, mTRPV4: 5.9 μM, rTRPV4: 3.2 μM). RN-1734 clearly decreases the production of tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) without altering the number of olig2-positive cells.

For research use only. We do not sell to patients.

RN-1734 Chemical Structure

RN-1734 Chemical Structure

CAS No. : 946387-07-1

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Free Sample (0.5-1 mg)   Apply Now  
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
5 mg USD 60 In-stock
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10 mg USD 84 In-stock
Estimated Time of Arrival: December 31
50 mg USD 384 In-stock
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100 mg USD 672 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products

    RN-1734 purchased from MCE. Usage Cited in: Acs Biomater Sci Eng. 2019 Dec 9;5(12):6520-6529.

    Protein levels of NFATc1 in nuclei of BMSCs cultured on various surfaces with or without GSK101 and RN-1734

    RN-1734 purchased from MCE. Usage Cited in: Acs Biomater Sci Eng. 2019 Dec 9;5(12):6520-6529.

    Effects of TRPV4 on NFATc1 nuclear localization in BMSCs on MNTs. Micrographs stained for NFATc1 of BMSCs cultured for 7d on (A) PT, (B) PT with TRPV4 agonist (GSK101) , (C) TNT-30, (D)TNT-100, (E) TNT-100 with TRPV4 antagonist (RN-1734). Red fluorescence: NFATc1; Blue fluorescence: nucleus.

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    Description

    RN-1734 is selective antagonist of the TRPV4 channel, completely antagonizes 4αPDD-mediated activation of TRPV4 with comparable, low micromolar IC50s for all three species (hTRPV4: 2.3 μM, mTRPV4: 5.9 μM, rTRPV4: 3.2 μM)[1]. RN-1734 clearly decreases the production of tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) without altering the number of olig2-positive cells[2].

    IC50 & Target

    IC50: 2.3 μM (hTRPV4), 5.9 μM (mTRPV4), 3.2 μM (rTRPV4)[1]

    In Vitro

    RN-1734 (27 hours; 10μM) reverses the increase in the apoptotic rate of oligodendrocytes induced by CM (LPS-activated microglia group) apoptosis[2].
    RN-1734 (27 hours; 10μM) alleviates CM-induced decreases in CNP[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Apoptosis Analysis[2]

    Cell Line: Microglial cells
    Concentration: 27 hours
    Incubation Time: 10μM
    Result: Significantly decreased the percentage of cleaved-caspase 3 positive cells.

    Western Blot Analysis[2]

    Cell Line: Microglial cells
    Concentration: 27 hours
    Incubation Time: 10 μM
    Result: Alleviated CM (with LPS only)-induced decreases in CNP.
    In Vivo

    RN-1734 (0.5 μl; microinjector pump; daily for 5 weeks) significantly reverses the decrease in CNP protein and improves myelination in CPZ-induced demyelination mouse[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: CPZ-induced demyelination mouse model (C57BL/6 male mice)[2]
    Dosage: 0.5 μl (10 μM in 5% DMSO and 0.9% NaCl)
    Administration: Microinjector pump for 5 weeks
    Result: Significantly reversed the decrease in CNP protein and improved myelination in CPZ-induced demyelination mouse.
    Molecular Weight

    353.31

    Formula

    C₁₄H₂₂Cl₂N₂O₂S

    CAS No.

    946387-07-1

    SMILES

    O=S(C1=CC=C(Cl)C=C1Cl)(N(C(C)C)CCNC(C)C)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (141.52 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8304 mL 14.1519 mL 28.3038 mL
    5 mM 0.5661 mL 2.8304 mL 5.6608 mL
    10 mM 0.2830 mL 1.4152 mL 2.8304 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 3.25 mg/mL (9.20 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 3.25 mg/mL (9.20 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 3.25 mg/mL (9.20 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
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    Keywords:

    RN-1734RN1734RN 1734TRP ChannelTransient receptor potential channelsInhibitorinhibitorinhibit

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