1. Membrane Transporter/Ion Channel
    Neuronal Signaling
  2. TRP Channel
  3. RN-1734

RN-1734 

Cat. No.: HY-19975 Purity: 99.01%
Handling Instructions

RN-1734 is selective antagonist of the TRPV4 channel, completely antagonizes 4αPDD-mediated activation of TRPV4 with comparable, low micromolar IC50s for all three species (hTRPV4: 2.3 μM, mTRPV4: 5.9 μM, rTRPV4: 3.2 μM). RN-1734 clearly decreases the production of tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) without altering the number of olig2-positive cells.

For research use only. We do not sell to patients.

RN-1734 Chemical Structure

RN-1734 Chemical Structure

CAS No. : 946387-07-1

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
5 mg USD 60 In-stock
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10 mg USD 84 In-stock
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50 mg USD 384 In-stock
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100 mg USD 672 In-stock
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Customer Review

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products

    RN-1734 purchased from MCE. Usage Cited in: Acs Biomater Sci Eng. 2019 Nov.

    Protein levels of NFATc1 in nuclei of BMSCs cultured on various surfaces with or without GSK101 and RN-1734

    RN-1734 purchased from MCE. Usage Cited in: Acs Biomater Sci Eng. 2019 Nov.

    Effects of TRPV4 on NFATc1 nuclear localization in BMSCs on MNTs. Micrographs stained for NFATc1 of BMSCs cultured for 7d on (A) PT, (B) PT with TRPV4 agonist (GSK101) , (C) TNT-30, (D)TNT-100, (E) TNT-100 with TRPV4 antagonist (RN-1734). Red fluorescence: NFATc1; Blue fluorescence: nucleus.
    • Biological Activity

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    Description

    RN-1734 is selective antagonist of the TRPV4 channel, completely antagonizes 4αPDD-mediated activation of TRPV4 with comparable, low micromolar IC50s for all three species (hTRPV4: 2.3 μM, mTRPV4: 5.9 μM, rTRPV4: 3.2 μM)[1]. RN-1734 clearly decreases the production of tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) without altering the number of olig2-positive cells[2].

    IC50 & Target

    IC50: 2.3 μM (hTRPV4), 5.9 μM (mTRPV4), 3.2 μM (rTRPV4)[1]

    In Vitro

    RN-1734 (27 hours; 10μM) reverses the increase in the apoptotic rate of oligodendrocytes induced by CM (LPS-activated microglia group) apoptosis[2].
    RN-1734 (27 hours; 10μM) alleviates CM-induced decreases in CNP[2].

    Apoptosis Analysis[2]

    Cell Line: Microglial cells
    Concentration: 27 hours
    Incubation Time: 10μM
    Result: Significantly decreased the percentage of cleaved-caspase 3 positive cells.

    Western Blot Analysis[2]

    Cell Line: Microglial cells
    Concentration: 27 hours
    Incubation Time: 10 μM
    Result: Alleviated CM (with LPS only)-induced decreases in CNP.
    In Vivo

    RN-1734 (0.5 μl; microinjector pump; daily for 5 weeks) significantly reverses the decrease in CNP protein and improves myelination in CPZ-induced demyelination mouse[2].

    Animal Model: CPZ-induced demyelination mouse model (C57BL/6 male mice)[2]
    Dosage: 0.5 μl (10 μM in 5% DMSO and 0.9% NaCl)
    Administration: Microinjector pump for 5 weeks
    Result: Significantly reversed the decrease in CNP protein and improved myelination in CPZ-induced demyelination mouse.
    Molecular Weight

    353.31

    Formula

    C₁₄H₂₂Cl₂N₂O₂S

    CAS No.

    946387-07-1

    SMILES

    O=S(C1=CC=C(Cl)C=C1Cl)(N(C(C)C)CCNC(C)C)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (141.52 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8304 mL 14.1519 mL 28.3038 mL
    5 mM 0.5661 mL 2.8304 mL 5.6608 mL
    10 mM 0.2830 mL 1.4152 mL 2.8304 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 3.25 mg/mL (9.20 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 3.25 mg/mL (9.20 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 3.25 mg/mL (9.20 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
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    Keywords:

    RN-1734RN1734RN 1734TRP ChannelTransient receptor potential channelsInhibitorinhibitorinhibit

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    Product name:
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    Cat. No.:
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