1. Metabolic Enzyme/Protease
    Autophagy
    Anti-infection
  2. Cytochrome P450
    Autophagy
    Virus Protease
  3. Schisandrin A

Schisandrin A (Synonyms: Schizandrin-A; Wuweizisu-A; Deoxyschizandrin)

Cat. No.: HY-N0693 Purity: 99.67%
Handling Instructions

Schisandrin A inhibits CYP3A activity with an IC50 of 6.60 μM and Ki of 5.83 μM, respectively.

For research use only. We do not sell to patients.

Schisandrin A Chemical Structure

Schisandrin A Chemical Structure

CAS No. : 61281-38-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 86 In-stock
Estimated Time of Arrival: December 31
10 mg USD 78 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of Schisandrin A:

Top Publications Citing Use of Products

    Schisandrin A purchased from MCE. Usage Cited in: Eur J Pharmacol. 2019 Jul 15;855:10-19.

    Western analysis of Cyclin D1 and Cdk-4 expression in HCC827GR cells with the treatment of SchA , Gefi and the combination, respectively.

    Schisandrin A purchased from MCE. Usage Cited in: Eur J Pharmacol. 2019 Jul 15;855:10-19.

    Western analysis of Cleaved-PARP, Cleaved-Caspase3 and Bcl2 expression in HCC827GR cells with the treatment of SchA , Gefi and the combination, respectively.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Schisandrin A inhibits CYP3A activity with an IC50 of 6.60 μM and Ki of 5.83 μM, respectively.

    IC50 & Target

    CYP3A

    6.6 μM (IC50)

    Autophagy

     

    In Vitro

    Schisandrin A (Sch A) strongly inhibits microsomal midazolam 1-hydroxylation catalyzed by CYP3A, with an IC50 of 6.60 μM. The recovery of enzyme activity in the absence or presence of Schisandrin A is shown in dilution assay plots. The Ki value for Schisandrin A is obtained from the Dixon plots and is 5.83 μM. The inactivation of rat liver microsomal midazolam 1-hydroxylation activity by Schisandrin A in the presence of NADPH is found to be time- and concentration-dependent. The Kinact and Ki are estimated to be 0.134/min and 4.51 μM, respectively for Schisandrin A[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Schisandrin A (SchA) significantly inhibits CYP3A activity in rat hepatic microsomes and Vmax value of each group in a concentration-dependent manner. The double-reciprocal plots and the secondary plot show that Schisandrin A inhibits CYP3A activity, with an apparent Ki value of 30.67 mg/kg. In each Schisandrin A-treated group, Schisandrin A also significantly decreases 1-hydroxymidazolam plasma concentrations compared with the negative group (to levels similar to the positive group)[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    416.51

    Formula

    C₂₄H₃₂O₆

    CAS No.
    SMILES

    C[[email protected]]1CC2=CC(OC)=C(OC)C(OC)=C2C3=C(OC)C(OC)=C(OC)C=C3C[[email protected]]1C

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (120.05 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4009 mL 12.0045 mL 24.0090 mL
    5 mM 0.4802 mL 2.4009 mL 4.8018 mL
    10 mM 0.2401 mL 1.2005 mL 2.4009 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (6.00 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 2.5 mg/mL (6.00 mM); Suspended solution; Need ultrasonic

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (6.00 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Kinase Assay
    [1]

    For the inactivation of CYP3A4 activity, microsomes are preincubated with inhibitors (Schisandrin A, 2.4 μM, 7.2 μM and 12.0 μM; or Sch B) at 37°C for up to 15 min in the presence of NADPH. Reactions are initiated with the addition of substrate midazolam and incubated at 37°C for 10 min. The enzyme inactivation is analyzed. Duplicates are prepared and tested[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [2]

    Rats[2]
    Healthy male Sprague-Dawley rats, weighing 250-280 g and 2-3 months of age, are used. The rats are randomly divided into five groups with 16 rats in each group. The animals are administered once daily for three consecutive days. The Schisandrin A-treated groups are administered intragastrically with doses of 32, 16 or 8 mg/kg of Schisandrin A (physiological saline as vehicle), and the rats are similarly administered with equal volume of vehicle in the negative control group and Ketoconazole (75 mg/kg) in the positive control group. All animals are allowed free access to food but are fasted overnight before scarification to reduce the intestinal content, and each group is randomly divided into two parts with eight rats in each part[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 99.67%

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    Keywords:

    Schisandrin ASchizandrin-A Wuweizisu-A DeoxyschizandrinCytochrome P450AutophagyVirus ProteaseCYPsInhibitorinhibitorinhibit

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