Anti-Human/Mouse EGFR Antibody (7A7) 

Anti-Human/Mouse EGFR Antibody (7A7) ia an anti-mouse EGFR monoclonal antibody proposed to be the mouse equivalent of cetuximab. Anti-Human/Mouse EGFR Antibody (7A7) fails to induce tumor regression in the HPV38 tumor model where EGFR is not expressed in mice. Anti-Human/Mouse EGFR Antibody (7A7) can be used in EGFR-expressing HPV38 tumor-related studies^[1].

Mouse IgG2a kappa

Mouse IgG2a kappa, Isotype Control

Human/Mouse

7A7 (60 mins at room temperature) exhibits non-specific intracellular and plasma membrane staining in EGFR-positive A431, 3T3-L1, and HPV38 cells, and EGFR-negative TC-1 cells, indicating it does not specifically detect native EGFR via immunofluorescence^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

7A7 (56 μg; i.v.; every three days) does not reduce tumour growth or improve survival in C57BL/6 mice bearing subcutaneous TC-1 squamous cell carcinoma tumours^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1956  [NCBI]

P00533

ERBB1/EGFR/HER1

ELISA, FACS, Functional assay, Research in vivo

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

145.56 kDa

[Anti-Human/Mouse EGFR Antibody (7A7)]

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. He X, et al. Characterization of 7A7, an anti-mouse EGFR monoclonal antibody proposed to be the mouse equivalent of cetuximab. Oncotarget. 2018 Jan 13;9(15):12250-12260.  [Content Brief]