Anti-NKG2D/CD314 Antibody (1D11) 

Anti-NKG2D/CD314 Antibody (1D11) is a kind of mouse IgG1 κ chimeric antibody, targeting to human NKG2D/CD314. Anti-NKG2D/CD314 Antibody (1D11) can block the binding of NKG2D to its ligands. Anti-NKG2D/CD314 Antibody (1D11) can be used for the research of infection, such as HIV (human immunodeficiency virus)^[1].

Mouse IgG1 kappa

Mouse IgG1 kappa, Isotype Control

Human

NKG2D/CD314

Anti-NKG2D/CD314 Antibody (1D11) (5 μg/mL, 4 h) reduces antibody-dependent cell-mediated cytotoxicity (ADCC) in gp120-pulsed CEM.NKr-CCR5 target cells^[1].
Anti-NKG2D/CD314 Antibody (1D11) (5 μg/mL, 4 h) reduces anti-HIV (human immunodeficiency virus)-1 ADCC of CEM.NKr-CCR5 target cells infected with U-N- HIV-1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

27007  [NCBI]

O54709

in vitro NKG2D blocking; Immunoprecipitation; Flow cytometry

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

150 kDa

Liquid

Colorless to light yellow

[Anti-NKG2D/CD314 Antibody (1D11)]

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Flow Cytometry analysis of Ramos cells labelling CD314 (red) with Anti-NKG2D/CD314 Antibody (HY-P990829). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG1 kappa (HY-P99977, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Parsons MS, et al. NKG2D Acts as a Co-Receptor for Natural Killer Cell-Mediated Anti-HIV-1 Antibody-Dependent Cellular Cytotoxicity. AIDS Res Hum Retroviruses. 2016 Oct/Nov;32(10-11):1089-1096.  [Content Brief]