AF647-conjugated CD235a Antibody (HI264)
(Synonyms: CD235a,CD235a-AF647,HI264)AF647-conjugated CD235a Antibody (HI264) is a Mouse-derived and AF647 conjugated monoclonal antibody, targeting to CD235a.
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Host:
Mouse
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Isotype:
Mouse IgG2a,κ
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Application:
FC
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Reactivity :
Human
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Formulation:
Supplied in PBS (pH 7.2) containing 0.1% Proclin300 and protein stabilizer.
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Conjugation:
AF647
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
FC
FC: Flow Cytometry
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|---|---|
| Dilution Ratio | 5 ul per 106 cells in a 100 µl suspension |
Product Details
AF647-conjugated CD235a Antibody (HI264) is a Mouse-derived and AF647 conjugated monoclonal antibody, targeting to CD235a.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
AF647
Unmodified
Mouse IgG2a,κ
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.2) containing 0.1% Proclin300 and protein stabilizer.
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Storage & Stability
Stored at 2-8°C for 1 year, protect from light, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
CD235a (Glycophorin A, GYPA) is a highly glycosylated membrane protein found on the surface of erythrocytes and is one of the most classic markers for erythroid cells. CD235a is primarily expressed on mature erythrocytes and erythroid progenitor cells; it is used for erythrocyte identification, the study of erythroid differentiation, and research into anemia and hematological disorders.
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein
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Isoforms & Post-Translational Modification
CD235a has 3 isomers: P02724-1: 16430 Da (predicted); P02724-2: 13649 Da (predicted); P02724-3: 13000 Da (predicted).
The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific) -
Subunit
Homodimer. Component of the ankyrin-1 complex in the erythrocyte, composed of ANK1, RHCE, RHAG, SLC4A1, EPB42, GYPA, GYPB and AQP1. Interacts with SLC4A1; a GYPA monomer is bound at each end of the SLC4A1 dimer forming a heterotetramer
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Synonyms
CD235a,CD235a-AF647,HI264
Documentation
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