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  5. AQP8 Antibody (YA8658)(PBS only)

AQP8 Antibody (YA8658)(PBS only)

Cat. No.: HY-P88974A
User Guide for Antibodies Technical Support

AQP8 Antibody (YA8658) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to AQP8.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

  • References

Description

AQP8 Antibody (YA8658) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to AQP8.
Host

Mouse
Molecular Weight

Predicted band size: 27.2 kDa
Species Reactivity
Human
SwissProt ID
Immunogen

Full length human recombinant protein of human AQP8 produced in HEK293T.
Application &
Dilution Ratio
Application Dilution Ratio
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:2000-2500
Purity Affinity purified Conjugation Non-conjugated
Isotype IgG1  
Appearance

Liquid
Formulation

Supplied in PBS, pH 7.4.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Channel that allows the facilitated permeation of water and uncharged molecules, such as hydrogen peroxide and the neutral form of ammonia (NH3), through cellular membranes such as plasma membrane, inner mitochondrial membrane and endoplasmic reticulum membrane of several tissues (PubMed:15948717, PubMed:18948439, PubMed:23541115, PubMed:26972385, PubMed:29732408, PubMed:30579780). The transport of the ammonia neutral form induces a parallel transport of proton, at alkaline pH when the concentration of ammonia is high (By similarity). However, it is unclear whether the transport of proton takes place via the aquaporin or via an endogenous pathway (By similarity). Also, may transport ammonia analogs such as formamide and methylamine, a transport favourited at basic pH due to the increase of unprotonated (neutral) form, which is expected to favor diffusion (PubMed:15948717). Does not transport urea or glycerol (PubMed:15948717). The water transport mechanism is mercury- and copper-sensitive and passive in response to osmotic driving forces (PubMed:15948717). At the canicular plasma membrane, mediates the osmotic transport of water toward the bile canaliculus and facilitates the cAMP-induced bile canalicular water secretion, a process involved in bile formation (PubMed:18948439). In addition, mediates the hydrogen peroxide release from hepatocyte mitochondria that modulates the SREBF2-mediated cholesterol synthesis and facilitates the mitochondrial ammonia uptake which is metabolized into urea, mainly under glucagon stimulation (PubMed:30579780, PubMed:34292591). In B cells, transports the CYBB-generated hydrogen peroxide from the external leaflet of the plasma membrane to the cytosol to promote B cell activation and differentiation for signal amplification (By similarity). In the small intestine and colon system, mediates water transport through mitochondria and apical membrane of epithelial cells (By similarity). May play an important role in the adaptive response of proximal tubule cells to acidosis possibly by facilitating the mitochondrial ammonia transport (PubMed:22622463)
Subcellular Localization：Cell membrane,Mitochondrion inner membrane,Apical cell membrane,Basolateral cell membrane,Smooth endoplasmic reticulum membrane
Expression：
Tissue_Specificity: Detected in the sperm midpiece (at protein level) (PubMed:28042826). Expressed only in pancreas and colon
Induction: Regulated by osmotic stress (PubMed:20144166). Up-regulated in response to metabolic acidosis (PubMed:22622463)
Isoforms & Post-Translational Modification：O94778: 261 amino acids, molecular weight 27381 Da.
Synonyms
AQP-8
Documentation
User Guide for Antibodies (1077 KB)
References

AQP8 Antibody (YA8658)(PBS only) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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