CD22 Antibody (YA1267)
(Synonyms: CD22; SIGLEC2; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD antigen CD22)CD22 Antibody (YA1267) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD22.
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Host:
Rabbit
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Isotype:
IgG
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Application:
IHC-P
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Reactivity :
Human
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|
| Dilution Ratio | 1:100-1:200 |
Product Details
CD22 Antibody (YA1267) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD22.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 130 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 95 kDa
Recombinant protein of human CD22
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD22 (Siglec 2) is a 135-kDa B-cell restricted antigen, and is an inhibitory coreceptor of the B-cell receptor (BCR) . The extracellular portion of CD22 comprises seven immunoglobulin domains, and is responsible for binding α2,6Sia ligands. Interaction with these ligands in cis (on the B-cell surface) regulates the association of CD22 with the BCR and thereby modulates the inhibitory function of CD22. Interaction of CD22 to ligands in trans (on the surface of other cells) can regulate both B-cell migration as well as the BCR signaling threshold.
CD22 can also recruit the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits BCR-induced Ca2+ signaling on normal B cells. CD22 is critical in maintaining a baseline level of B-cell inhibition and homeostasis in humoral immunity. CD22 is a target molecule in dysregulated B cells that cause autoimmune diseases and blood cancers[1][2]. -
Subcellular Localization
Cell membrane; Single-pass type I membrane protein
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Expression
Tissue_specificity:B lymphocytes -
Isoforms & Post-Translational Modification
P20273 has 5 isomers: P20273-1: 95348 Da (predicted); P20273-2: 75799 Da (predicted); P20273-3: 85675 Da (predicted); P20273-4: 84480 Da (predicted); P20273-5: 75354 Da (predicted).
Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2;Phosphorylated on tyrosine residues by LYN -
Subunit
Predominantly monomer of isoform CD22-beta. Also found as heterodimer of isoform CD22-beta and a shorter isoform. Interacts with PTPN6/SHP-1, LYN, SYK, PIK3R1/PIK3R2 and PLCG1 upon phosphorylation. Interacts with GRB2, INPP5D and SHC1 upon phosphorylation (By similarity). May form a complex with INPP5D/SHIP, GRB2 and SHC1
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SwissProt ID
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Synonyms
CD22; SIGLEC2; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD antigen CD22
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Research Field
Immunology
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