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CD79b Antibody (YA3995)

(Synonyms: B29; IGB; AGM6)
Customer Review

Based on 1 Customer Validation

CD79b Antibody (YA3995) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD79b.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD79b Antibody (YA3995)
  • Experimental Validation Results for CD79b Antibody (YA3995)
  • CD79b Antibody (YA3995) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:25-1:50 1:200-1:400 1:10000

Product Details

Description

CD79b Antibody (YA3995) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD79b.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 30-50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 26 kDa
Immunogen

Purified recombinant fragment of human CD79B (AA:extra 30-160) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD79b Antibody (YA3995)
    Immunocytochemistry analysis of Hela cells labeling CD79b Antibody (YA3995) with CD79b Antibody (YA3995) (HY-P84298) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD79b Antibody (YA3995) ((HY-P84298) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CD79b Antibody (YA3995)
    Immunocytochemistry analysis of Hela cells labeling CD79b Antibody (YA3995) with CD79b Antibody (YA3995) (HY-P84298) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD79b Antibody (YA3995) ((HY-P84298) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    CD79b is required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:B cells

  • Isoforms & Post-Translational Modification

    P40259 has 3 isomers: P40259-1: 26048 Da (predicted); P40259-2: 14030 Da (predicted); P40259-3: 26119 Da (predicted).
    Phosphorylated on tyrosine upon B-cell activation by SRC-type Tyr-kinases such as BLK, LYN and SYK

  • Subunit

    Heterodimer of alpha and beta chains; disulfide-linked. Part of the B-cell antigen receptor complex where the alpha/beta chain heterodimer is non-covalently associated with an antigen-specific membrane-bound surface immunoglobulin of two heavy chains and two light chains (PubMed:35981043).

  • SwissProt ID

    P40259

  • Gene ID
    974 [NCBI]
  • Synonyms

    B29; IGB; AGM6

CD79b Antibody (YA3995) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling CD79b Antibody (YA3995) with CD79b Antibody (YA3995) (HY-P84298) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD79b Antibody (YA3995) ((HY-P84298) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling CD79b Antibody (YA3995) with CD79b Antibody (YA3995) (HY-P84298) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD79b Antibody (YA3995) ((HY-P84298) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CD79b Antibody (YA3995) Product Image
    1/16
  • Experimental Validation Results for CD79b Antibody (YA3995)
  • Experimental Validation Results for CD79b Antibody (YA3995)
  • CD79b Antibody (YA3995) Product Image
  • Experimental Validation Results for CD79b Antibody (YA3995)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling CD79b Antibody (YA3995) with CD79b Antibody (YA3995) (HY-P84298) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD79b Antibody (YA3995) ((HY-P84298) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CD79b Antibody (YA3995)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling CD79b Antibody (YA3995) with CD79b Antibody (YA3995) (HY-P84298) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD79b Antibody (YA3995) ((HY-P84298) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CD79b Antibody (YA3995)
  • Experimental Validation Results for CD79b Antibody (YA3995)
  • CD79b Antibody (YA3995) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling CD79b Antibody (YA3995) with CD79b Antibody (YA3995) (HY-P84298) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD79b Antibody (YA3995) ((HY-P84298) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CD79b Antibody (YA3995) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling CD79b Antibody (YA3995) with CD79b Antibody (YA3995) (HY-P84298) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD79b Antibody (YA3995) ((HY-P84298) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CD79b Antibody (YA3995) Product Image ICC
  • CD79b Antibody (YA3995) Product Image ICC