DDR2 Antibody (YA4319)
(Synonyms: TKT; MIG20a; NTRKR3; TYRO10)
Based on 1 Customer Validation
DDR2 Antibody (YA4319) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to DDR2.
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Host:
Mouse
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Isotype:
IgG2a
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Application:
IHC-P, ICC/IF, FC, ELISA
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Reactivity :
Human, Mouse, Rat, Rabbit
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Formulation:
Supplied in PBS with 0.05% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
DDR2 Antibody (YA4319) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to DDR2.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat, Rabbit
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Observed Molecular WeightObserved band size: 97 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 97 kDa
Purified recombinant fragment of human DDR2 aa 422-639.
affinity purified.
Non-conjugated
Unmodified
IgG2a
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Flow cytometric analysis of 1X10^6 NIH3T3 cells labeling DDR2 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).
Background
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Function
DDR2 (discoidin domain receptor 2) is a collagen-activated receptor tyrosine kinase that regulates cell-collagen interactions in normal and pathological tissue contexts[1]. Mechanistically, type I collagen activates DDR2 and promotes Src and Shc signaling, linking extracellular matrix sensing to intracellular phosphorylation responses[2]. In cartilage disease models, DDR2 activation induces MMP-13, connecting collagen receptor signaling with matrix degradation in osteoarthritis-related cartilage damage[3]. In breast cancer models, collagen I-activated DDR2 stabilizes SNAIL1 through Src-dependent ERK2 activity, supporting invasion, migration, epithelial-mesenchymal transition, and metastasis[4]. Compared with related isoforms, DDR1 is mainly associated with epithelial cells, whereas DDR2 is mainly associated with mesenchymal cells, making DDR2 especially relevant to fibroblast-rich stroma, cartilage, and collagen-dense tumor microenvironments[1]. In lung squamous cell carcinoma, DDR2 kinase mutations occur in a defined patient subset and confer sensitivity to Dasatinib (HY-10181) in experimental models[5]. For experimental applications, the extracellular allosteric inhibitor WRG-28 blocks DDR2-collagen interaction, inhibits tumor invasion and migration, and reduces metastatic breast tumor cell colonization in lungs[6].
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein
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Expression
Tissue_specificity:Protein levels were detected in osteocytes, subchondral osteoblasts, bone lining cells, tibia, and cartilage. Higher levels were found in the heart and lungs, and lower levels in the brain, placenta, liver, skeletal muscle, pancreas, and kidneys.
Induction:Up-regulated during osteoblast differentiation (in vitro) . Up-regulated in cartilage from osteoarthritis -
Subunit
Binds hydroxyproline-rich sequence motifs in fibrillar, glycosylated collagen, such as the GQOGVMGFO motif, where O stands for hydroxyproline. Interacts with SRC. Interacts (tyrosine phosphorylated) with SHC1
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SwissProt ID
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Synonyms
TKT; MIG20a; NTRKR3; TYRO10
Documentation
References
[1]. Fu HL, et al. Discoidin domain receptors: unique receptor tyrosine kinases in collagen-mediated signaling. J Biol Chem. 2013 Mar 15;288(11):7430-7437. [Content Brief]
[2]. Ikeda K, et al. Discoidin domain receptor 2 interacts with Src and Shc following its activation by type I collagen. J Biol Chem. 2002 May 24;277(21):19206-12. [Content Brief]
[3]. Xu L, et al. Activation of the discoidin domain receptor 2 induces expression of matrix metalloproteinase 13 associated with osteoarthritis in mice. J Biol Chem. 2005 Jan 7;280(1):548-55. [Content Brief]
[4]. Zhang K, et al. The collagen receptor discoidin domain receptor 2 stabilizes SNAIL1 to facilitate breast cancer metastasis. Nat Cell Biol. 2013 Jun;15(6):677-87. [Content Brief]
[5]. Hammerman PS, et al. Mutations in the DDR2 kinase gene identify a novel therapeutic target in squamous cell lung cancer. Cancer Discov. 2011 Jun;1(1):78-89. [Content Brief]
[6]. Grither WR, et al. Inhibition of tumor-microenvironment interaction and tumor invasion by small-molecule allosteric inhibitor of DDR2 extracellular domain. Proc Natl Acad Sci U S A. 2018 Aug 14;115(33):E7786-E7794. [Content Brief]
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