HBS1L Antibody (YA8203)(PBS only)

Cat. No.: HY-P88519A: Data Sheet User Guide for Antibodies
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HBS1L Antibody (YA8203) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to HBS1L.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

HBS1L Antibody (YA8203) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to HBS1L.

Host

Mouse

Molecular Weight

Predicted band size: 75.3 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9Y450

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-260 of human HBS1L produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:500-800

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：GTPase component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway (PubMed:21448132, PubMed:23667253, PubMed:27863242). The Pelota-HBS1L complex recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel (PubMed:27863242). Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway (PubMed:21448132, PubMed:32006463)

Subcellular Localization：Cytoplasm,Cytoplasm

Expression：
Tissue_Specificity: Detected in heart, brain, placenta, liver, muscle, kidney and pancreas

Isoforms & Post-Translational Modification：Q9Y450 has three isomers: Q9Y450-1: 75473 Da (predicted); Q9Y450-2: 70128 Da (predicted); Q9Y450-4: 70625 Da (predicted).

Subunit：Component of the Pelota-HBS1L complex, also named Dom34-Hbs1 complex, composed of PELO and HBS1L (PubMed:27863242)

Synonyms

EF-1a; eRF3c; ERFS; HBS1; HSPC276

Documentation

User Guide for Antibodies (1077 KB)