HTR3A Antibody (YA4037)

MedChemExpress Validation
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using HTR3A Antibody (YA4037). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody（HY-P84340, 1/1000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

MedChemExpress Validation
Immunohistochemical analysis of paraffin-embedded human prostate tissue using HTR3A Antibody (YA4037). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody（HY-P84340, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

MedChemExpress Validation
Immunohistochemical analysis of paraffin-embedded human bladder cancer cancer tissue using HTR3A Antibody (YA4037). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody（HY-P84340, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

MedChemExpress Validation
Immunohistochemical analysis of paraffin-embedded human tonsils tissue using HTR3A Antibody (YA4037). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody（HY-P84340, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

MedChemExpress Validation
Immunofluorescence analysis of Hela cells labeling HTR3A antibody (HY-P84340) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

MedChemExpress Validation
Immunocytochemistry analysis of Hela cells labeling HTR3A with HTR3A Antibody (HY-P84340) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with HTR3A Antibody (HY-P84340) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

MedChemExpress Validation
Immunocytochemistry analysis of Hela cells labeling HTR3A with HTR3A Antibody (HY-P84340) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with HTR3A Antibody (HY-P84340) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

MedChemExpress Validation
Flow cytometric analysis of 1X10^6 Hela cells labeling HTR3A Antibody(HY-P84340