IP3 receptor Antibody (YA7280)

Cat. No.: HY-P87596: Data Sheet User Guide for Antibodies
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IP3 receptor Antibody (YA7280) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IP3 receptor.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

IP3 receptor Antibody (YA7280) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IP3 receptor.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 314 kDa;

Observed band size: 314 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q14643

Immunogen

A synthesized peptide derived from human ITPR1

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:100-500
IP IP: Immunoprecipitation	1:20-50

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Inositol 1,4,5-trisphosphate-gated calcium channel that, upon inositol 1,4,5-trisphosphate binding, mediates calcium release from the endoplasmic reticulum (ER) (PubMed:10620513, PubMed:27108797). Undergoes conformational changes upon ligand binding, suggesting structural flexibility that allows the channel to switch from a closed state, capable of interacting with its ligands such as 1,4,5-trisphosphate and calcium, to an open state, capable of transferring calcium ions across the ER membrane (By similarity). Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CAMK2 complex (By similarity). Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1 (By similarity). Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix (By similarity). Regulates fertilization and egg activation by tuning the frequency and amplitude of calcium oscillations (By similarity)

Subcellular Localization：Endoplasmic reticulum membrane,Cytoplasmic vesicle, secretory vesicle membrane,Cytoplasm, perinuclear region

Expression：
Tissue_Specificity: Widely expressed

Isoforms & Post-Translational Modification：Q14643 has 8 isomers: Q14643-1: 313929 Da (predicted); Q14643-2: 312163 Da (predicted); Q14643-3: 308539 Da (predicted); Q14643-4: 306773 Da (predicted); Q14643-5: 311135 Da (predicted); Q14643-6: 312003 Da (predicted); Q14643-7: 310237 Da (predicted); Q14643-8: 309209 Da (predicted).
Polyubiquitinated (By similarity)丨Phosphorylated by cAMP kinase (PKA) enhances calcium release (By similarity)丨Phosphorylated on tyrosine residues丨Palmitoylated by ZDHHC6 in immune cells, leading to regulation of ITPR1 stability and function

Subunit：Homotetramer (By similarity)

Documentation

User Guide for Antibodies (1077 KB)