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Integrin beta 5 Antibody (YA3841)

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Based on 1 Customer Validation

Integrin beta 5 Antibody (YA3841) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Integrin beta 5.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2a

  • Application:

    ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Integrin beta 5 Antibody (YA3841)
  • Experimental Validation Results for Integrin beta 5 Antibody (YA3841)
  • Integrin beta 5 Antibody (YA3841) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:50-1:200 1:200-1:400 1:10000

Product Details

Description

Integrin beta 5 Antibody (YA3841) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Integrin beta 5.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 88 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 88 kDa
Immunogen

Purified recombinant fragment of human ITGB5 (AA: 50-319) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2a

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Integrin beta 5 Antibody (YA3841)
    Immunocytochemistry analysis of A549 cells labeling Integrin beta 5 with Integrin beta 5 Antibody (HY-P84144) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin beta 5 Antibody (HY-P84144) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Integrin beta 5 Antibody (YA3841)
    Immunocytochemistry analysis of A549 cells labeling Integrin beta 5 with Integrin beta 5 Antibody (HY-P84144) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin beta 5 Antibody (HY-P84144) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Integrin beta 5 is an Integrin alpha-V/beta-5 (ITGAV:ITGB5) is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand; (Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for adenovirus type C

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Subunit

    Heterodimer of an alpha and a beta subunit. Beta-5 (ITGB5) associates with alpha-V (ITGAV). Interacts with MYO10 (PubMed:15156152). Interacts with DAB2.

  • SwissProt ID

    P18084

  • Gene ID
    3693 [NCBI]

Integrin beta 5 Antibody (YA3841) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Integrin beta 5 with Integrin beta 5 Antibody (HY-P84144) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin beta 5 Antibody (HY-P84144) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Integrin beta 5 with Integrin beta 5 Antibody (HY-P84144) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin beta 5 Antibody (HY-P84144) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Integrin beta 5 Antibody (YA3841) Product Image
    1/16
  • Experimental Validation Results for Integrin beta 5 Antibody (YA3841)
  • Experimental Validation Results for Integrin beta 5 Antibody (YA3841)
  • Integrin beta 5 Antibody (YA3841) Product Image
  • Experimental Validation Results for Integrin beta 5 Antibody (YA3841)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Integrin beta 5 with Integrin beta 5 Antibody (HY-P84144) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin beta 5 Antibody (HY-P84144) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Integrin beta 5 Antibody (YA3841)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Integrin beta 5 with Integrin beta 5 Antibody (HY-P84144) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin beta 5 Antibody (HY-P84144) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Integrin beta 5 Antibody (YA3841)
  • Experimental Validation Results for Integrin beta 5 Antibody (YA3841)
  • Integrin beta 5 Antibody (YA3841) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling Integrin beta 5 with Integrin beta 5 Antibody (HY-P84144) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin beta 5 Antibody (HY-P84144) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Integrin beta 5 Antibody (YA3841) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling Integrin beta 5 with Integrin beta 5 Antibody (HY-P84144) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin beta 5 Antibody (HY-P84144) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Integrin beta 5 Antibody (YA3841) Product Image ICC
  • Integrin beta 5 Antibody (YA3841) Product Image ICC