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  4. Integrin beta 5 Antibody (YA3841)

Integrin beta 5 Antibody (YA3841)

Cat. No.: HY-P84144
COA User Guide for Antibodies Technical Support

Integrin beta 5 Antibody (YA3841) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Integrin beta 5.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay
  • Product Detail

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  • Documentation

Description

Integrin beta 5 Antibody (YA3841) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Integrin beta 5.

Host

Mouse

Clonality

Monoclonal Antibody

Molecular Weight
Predicted band size: 88 kDa;
Observed band size: 88 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Purified recombinant fragment of human ITGB5 (AA: 50-319) expressed in E. Coli.

Application &
Dilution Ratio
Application Dilution Ratio
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
FC
FC: Flow Cytometry
1:200-1:400
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:10000
Purity affinity purified. Conjugation Non-conjugated
Modification Unmodified Isotype IgG2a
Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image
ICC
  • Immunocytochemistry analysis of A549 cells labeling Integrin beta 5 with Integrin beta 5 Antibody (HY-P84144) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin beta 5 Antibody (HY-P84144) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L(HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of A549 cells labeling Integrin beta 5 with Integrin beta 5 Antibody (HY-P84144) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin beta 5 Antibody (HY-P84144) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L(HY-P8005,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background
Function:Integrin alpha-V/beta-5 (ITGAV:ITGB5) is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand; (Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for adenovirus type C
Subcellular Localization:Cell membrane; Single-pass type I membrane protein
Subunit:Heterodimer of an alpha and a beta subunit. Beta-5 (ITGB5) associates with alpha-V (ITGAV). Interacts with MYO10 (PubMed:15156152). Interacts with DAB2.
Documentation

Integrin beta 5 Antibody (YA3841) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Integrin beta 5 Antibody (YA3841)
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HY-P84144
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