MCTS1 Antibody (YA7920)(PBS only)

Cat. No.: HY-P88236A: Data Sheet User Guide for Antibodies
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MCTS1 Antibody (YA7920) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to MCTS1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

MCTS1 Antibody (YA7920) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to MCTS1.

Host

Mouse

Molecular Weight

Predicted band size: 20.4 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9ULC4

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-181 of human MCTS1 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG2a

Appearance

Solution

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Translation regulator forming a complex with DENR to promote translation reinitiation. Translation reinitiation is the process where the small ribosomal subunit remains attached to the mRNA following termination of translation of a regulatory upstream ORF (uORF), and resume scanning on the same mRNA molecule to initiate translation of a downstream ORF, usually the main ORF (mORF). The MCTS1/DENR complex is pivotal to two linked mechanisms essential for translation reinitiation. Firstly, the dissociation of deacylated tRNAs from post-termination 40S ribosomal complexes during ribosome recycling. Secondly, the recruitment in an EIF2-independent manner of aminoacylated initiator tRNA to P site of 40S ribosomes for a new round of translation (PubMed:16982740, PubMed:20713520, PubMed:37875108). This regulatory mechanism governs the translation of more than 150 genes which translation reinitiation is MCTS1/DENR complex-dependent (PubMed:16982740, PubMed:20713520, PubMed:37875108). Consequently, modulates various unrelated biological processes including cell cycle regulation and DNA damage signaling and repair (PubMed:10440924, PubMed:11709712, PubMed:12637315, PubMed:15897892, PubMed:16322206, PubMed:17016429, PubMed:17416211, PubMed:9766643). Notably, it positively regulates interferon gamma immunity to mycobacteria by enhancing the translation of JAK2 (PubMed:37875108)

Subcellular Localization：Cytoplasm

Expression：
Tissue_Specificity: Ubiquitous. Over-expressed in T-cell lymphoid cell lines and in non-Hodgkin lymphoma cell lines as well as in a subset of primary large B-cell lymphomas
Induction: By DNA damaging agents such as gamma irradiation, adriamycin or taxol in lymphoid cells, but not by stress stimuli such as heat shock. This induction of protein expression does not occur at the RNA level, and does not require new protein synthesis

Isoforms & Post-Translational Modification：Q9ULC4 has three isomers: Q9ULC4-1: 20555 Da (predicted); Q9ULC4-2: 19229 Da (predicted); Q9ULC4-3: 20550 Da (predicted).
Phosphorylation is critical for stabilization and promotion of cell proliferation

Subunit：Interacts (via PUA domain) with DENR; the complex regulates translation reinitiation

Synonyms

MCT-1; MCT1

Documentation

User Guide for Antibodies (1077 KB)