MED15 Antibody (YA8649)

Cat. No.: HY-P88965: Data Sheet User Guide for Antibodies
FAQs
Technical Support

MED15 Antibody (YA8649) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MED15.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

MED15 Antibody (YA8649) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MED15.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 82.4 kDa

Species Reactivity

Human

SwissProt ID

Q96RN5

Immunogen

Human recombinant protein fragment corresponding to amino acids 393-679 of human MED15 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway

Subcellular Localization：Cytoplasm,Nucleus

Expression：
Tissue_Specificity: Expressed in all tissues examined, including heart, brain, lung, spleen, thymus, pancreas, blood leukocyte and placenta. However, the level of expression varied, with highest expression in the placenta and peripheral blood and lowest in the pancreas and kidney
Induction: By 12-O-tetradecanoylphorbol-13-acetate (TPA)

Isoforms & Post-Translational Modification：Q96RN5 has three isomers: Q96RN5-1: 86753 Da (predicted); Q96RN5-2: 82581 Da (predicted); Q96RN5-3: 75927 Da (predicted).
Ubiquitinated by TRIM11, leading to proteasomal degradation

Subunit：Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11

Synonyms

ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1; TNRC7

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/mda-mb-231-cell.html [Content Brief]