NFS1 Antibody (YA7971)

Cat. No.: HY-P88287: Ficha de datos User Guide for Antibodies
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NFS1 Antibody (YA7971) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to NFS1.

Para uso exclusivo en investigación. No vendemos a pacientes.

Tamaño	Precio	Stock
20 μL		Pide un presupuesto y un plazo de entrega
50 μL		Pide un presupuesto y un plazo de entrega
100 μL		Pide un presupuesto y un plazo de entrega

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn
Referencias

Descripciòn

NFS1 Antibody (YA7971) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to NFS1.

Host

Mouse

Clonality

Monoclonal

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9Y697

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-299 of human NFS1 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	Pureza	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2b

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Cysteine desulfurase, of the core iron-sulfur cluster (ISC) assembly complex, that catalyzes the desulfuration of L-cysteine to L-alanine, as component of the cysteine desulfurase complex, leading to the formation of a cysteine persulfide intermediate at the active site cysteine residue and participates in the [2Fe-2S] clusters assembly on the scaffolding protein ISCU (PubMed:18650437, PubMed:29097656, PubMed:31101807). The persulfide is then transferred on the flexible Cys loop from the catalytic site of NFS1 to the surface of NFS1 (PubMed:29097656). After the NFS1-linked persulfide sulfur is transferred to one of the conserved Cys residues of the scaffold, a reaction assisted by FXN (By similarity). The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (By similarity)|May catalyze the desulfuration of L-cysteine to L-alanine as component of the cysteine desulfurase complex (NFS1:LYRM4), leading to the formation of a cysteine persulfide intermediate (PubMed:16527810, PubMed:18650437). Acts as a sulfur donor for MOCS3 by transferring the sulfur of the cysteine persulfide intermediate on MOCS3 (PubMed:18650437, PubMed:23593335)

Subcellular Localization：Mitochondrion,Cytoplasm,Nucleus,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Expression：
Tissue_Specificity: Predominantly expressed in heart and skeletal muscle. Also found in brain, liver and pancreas

Isoforms & Post-Translational Modification：Q9Y697 has three isomers: Q9Y697-1: 50196 Da (predicted); Q9Y697-2: 44017 Da (predicted); Q9Y697-3: 44362 Da (predicted).
N-gluconoylated丨Cysteine persulfide intermediate is reduced by thiol-containing molecules like glutathione and L-cysteine

Subunit：Homodimer (PubMed:29097656, PubMed:31101807)

Synonyms

COXPD52; HUSSY-08; IscS; NIFS

Documentación

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/sf9-cell.html [Content Brief]