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NADPH Oxidase 4 Antibody (YA4513)

(Synonyms: KOX; KOX-1; RENOX)
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Based on 1 Customer Validation

NADPH Oxidase 4 Antibody (YA4513) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NADPH Oxidase 4.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4513)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4513)
  • NADPH Oxidase 4 Antibody (YA4513) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

NADPH Oxidase 4 Antibody (YA4513) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NADPH Oxidase 4.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 67 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 67 kDa
Immunogen

Purified recombinant fragment of human NOX4 (AA: 210-310) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718988

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4513)
    Immunocytochemistry analysis of HeLa cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P84816) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P84816) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4513)
    Immunocytochemistry analysis of HePG2 cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P84816) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P84816) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    NADPH Oxidase 4 is a NADPH oxidase that catalyzes predominantly the reduction of oxygen to H2O2. Can also catalyze to a smaller extent, the reduction of oxygen to superoxide. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Promotes ferroptosis, reactive oxygen species production and reduced glutathione (GSH) levels by activating NLRP3 inflammasome activation and cytokine release; NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. Involved in redox signaling in vascular cells. Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage; Lacks superoxide-generating NADPH oxidase activity[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16].

  • Subcellular Localization

    Cytoplasm; Endoplasmic reticulum membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein; Cell junction, focal adhesion; Nucleus; Nucleus; Nucleus, nucleolus; Cytoplasm; Cytoplasm, perinuclear region; Cytoplasm; Cytoplasm, perinuclear region; Cytoplasm; Cytoplasm, perinuclear region; Cytoplasm; Cytoplasm, perinuclear region

  • Expression


    Tissue_specificity:This gene is expressed (protein level) in distal renal tubular cells and endothelial cells of the renal cortex. Expression is widespread. It is highly expressed in the kidneys, and expressed at lower levels in the heart, adipocytes, liver cancer cells, endothelial cells, skeletal muscle, brain, various brain tumor cell lines, and airway epithelial cells.

    Induction:By 7-ketocholesterol (at protein level)

  • Isoforms & Post-Translational Modification

    Q9NPH5 has 9 isomers: Q9NPH5-1: 66932 Da (predicted); Q9NPH5-2: 58410 Da (predicted); Q9NPH5-3: 27625 Da (predicted); Q9NPH5-4: 31812 Da (predicted); Q9NPH5-5: 25761 Da (predicted); Q9NPH5-6: 62745 Da (predicted); Q9NPH5-7: 6516 Da (predicted); Q9NPH5-8: 64102 Da (predicted); Q9NPH5-9: 59915 Da (predicted).
    Deubiquitinated by USP19;N-glycosylated and glycosylation is required for its proper function;N-glycosylated

  • Subunit

    Interacts with protein disulfide isomerase (By similarity). Interacts with, relocalizes and stabilizes CYBA/p22phox. Interacts with TLR4. Interacts with PPP1R15A (PubMed:26742780). Interacts with LRRC8A; this interaction prevents the ubiquitin-mediated degradation of LRRC8A (PubMed:39909992)

  • SwissProt ID

    Q9NPH5

  • Gene ID
    50507 [NCBI]
  • Synonyms

    KOX; KOX-1; RENOX

NADPH Oxidase 4 Antibody (YA4513) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P84816) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P84816) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HePG2 cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P84816) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P84816) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NADPH Oxidase 4 Antibody (YA4513) Product Image
    1/16
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4513)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4513)
  • NADPH Oxidase 4 Antibody (YA4513) Product Image
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4513)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P84816) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P84816) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4513)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HePG2 cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P84816) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P84816) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4513)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4513)
  • NADPH Oxidase 4 Antibody (YA4513) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P84816) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P84816) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NADPH Oxidase 4 Antibody (YA4513) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HePG2 cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P84816) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P84816) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NADPH Oxidase 4 Antibody (YA4513) Product Image ICC
  • NADPH Oxidase 4 Antibody (YA4513) Product Image ICC