VHL Antibody (YA683)
(Synonyms: von Hippel-Lindau disease tumor suppressor, Protein G7, pVHL, VHL)VHL Antibody (YA683) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to VHL.
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Host:
Mouse
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Application:
IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|
| Dilution Ratio | 1:100-1:500 |
Product Details
VHL Antibody (YA683) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to VHL.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 24 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 24 kDa
Full length human recombinant protein of human VHL produced in HEK293T cell.
Endogenous
Immunogen affinity purified.
Non-conjugated
Unmodified
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Envío
Shipping with blue ice.
Background
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Function
VHL is involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2. Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR[1][2][3][4].
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Subcellular Localization
Cytoplasm; Cell membrane; Peripheral membrane protein; Endoplasmic reticulum; Nucleus; Cytoplasm; Nucleus
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Expression
Tissue_specificity:It is expressed in both the human brain and kidneys. -
Isoforms & Post-Translational Modification
P40337 has 3 isomers: P40337-1: 24153 Da (predicted); P40337-2: 19654 Da (predicted); P40337-3: 18532 Da (predicted).
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Subunit
Component of the VCB (VHL-Elongin BC-CUL2) complex; this complex acts as a ubiquitin-ligase E3 and directs proteasome-dependent degradation of targeted proteins.
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SwissProt ID
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Synonyms
von Hippel-Lindau disease tumor suppressor, Protein G7, pVHL, VHL
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Research Field
Cell Biology
Documentación
References
[1]. Tanimoto K, et al. Mechanism of regulation of the hypoxia-inducible factor-1 alpha by the von Hippel-Lindau tumor suppressor protein. EMBO J. 2000 Aug 15;19(16):4298-309. [Content Brief]
[2]. Cheng J, et al. SUMO-specific protease 1 is essential for stabilization of HIF1alpha during hypoxia. Cell. 2007 Nov 2;131(3):584-95. [Content Brief]
[3]. Xie L, et al. Oxygen-regulated beta(2)-adrenergic receptor hydroxylation by EGLN3 and ubiquitylation by pVHL. Sci Signal. 2009 Jul 7;2(78):ra33. [Content Brief]
[4]. Ganner A, et al. VHL suppresses RAPTOR and inhibits mTORC1 signaling in clear cell renal cell carcinoma. Sci Rep. 2021 Jul 21;11(1):14827. [Content Brief]
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