VHL Antibody (YA683)

Cat. No.: HY-P80517: Data Sheet User Guide for Antibodies
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VHL Antibody (YA683) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to VHL.

For research use only. We do not sell to patients.

Size	Price	Stock
20 μL		Ask For Quote & Lead Time
50 μL		Ask For Quote & Lead Time
100 μL		Ask For Quote & Lead Time

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VHL Antibody (YA683) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

VHL Antibody (YA683) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to VHL.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 24 kDa;

Observed band size: 24 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P40337

Gene ID

7428 [NCBI]

Immunogen

Synthesized peptide derived from human pVHL

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:500

Sensitivity	Endogenous	Purity	Immunogen affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in 1*PBS, 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex (PubMed:10944113, PubMed:17981124, PubMed:19584355). Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions (PubMed:10944113, PubMed:17981124). Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases (PubMed:10944113, PubMed:17981124). Ubiquitinates, in an oxygen-responsive manner, ADRB2 (PubMed:19584355). Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR (PubMed:34290272)

Subcellular Localization：Cytoplasm; Cell membrane; Peripheral membrane protein; Endoplasmic reticulum; Nucleus; Cytoplasm; Nucleus

Expression：
Tissue_specificity:It is expressed in both the human brain and kidneys.

Isoforms & Post-Translational Modification：P40337 has 3 isomers: P40337-1: 24153 Da (predicted); P40337-2: 19654 Da (predicted); P40337-3: 18532 Da (predicted).

Subunit：Component of the VCB (VHL-Elongin BC-CUL2) complex; this complex acts as a ubiquitin-ligase E3 and directs proteasome-dependent degradation of targeted proteins.

Database

Entrez Gene: 7428 Human

SwissProt: P40337 Human

OMIM: 171300 Human

Research Field

Cell Biology

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/u-251-cell.html [Content Brief]

VHL Antibody (YA683) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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