SH3BP1 Antibody (YA8375)(PBS only)

Cat. No.: HY-P88691A: Data Sheet User Guide for Antibodies
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SH3BP1 Antibody (YA8375) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SH3BP1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

SH3BP1 Antibody (YA8375) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SH3BP1.

Host

Mouse

Molecular Weight

Predicted band size: 75.5 kDa

Species Reactivity

Human, Mouse

SwissProt ID

Q9Y3L3

Immunogen

Full length human recombinant protein of human SH3BP1 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:500-800

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：GTPase activating protein (GAP) which specifically converts GTP-bound Rho-type GTPases including RAC1 and CDC42 in their inactive GDP-bound form. By specifically inactivating RAC1 at the leading edge of migrating cells, it regulates the spatiotemporal organization of cell protrusions which is important for proper cell migration (PubMed:21658605). Also negatively regulates CDC42 in the process of actin remodeling and the formation of epithelial cell junctions (PubMed:22891260). Through its GAP activity toward RAC1 and/or CDC42 plays a specific role in phagocytosis of large particles. Specifically recruited by a PI3 kinase/PI3K-dependent mechanism to sites of large particles engagement, inactivates RAC1 and/or CDC42 allowing the reorganization of the underlying actin cytoskeleton required for engulfment (PubMed:26465210). It also plays a role in angiogenesis and the process of repulsive guidance as part of a semaphorin-plexin signaling pathway. Following the binding of PLXND1 to extracellular SEMA3E it dissociates from PLXND1 and inactivates RAC1, inducing the intracellular reorganization of the actin cytoskeleton and the collapse of cells (PubMed:24841563)

Subcellular Localization：Cell projection,Cell junction, tight junction,Cell junction, adherens junction,Cell projection, phagocytic cup,Nucleus,Cytoplasm, cytosol

Isoforms & Post-Translational Modification：Q9Y3L3 has four isomers: Q9Y3L3-1: 75713 Da (predicted); Q9Y3L3-2: 50820 Da (predicted); Q6ZT62-1: 73599 Da (predicted); Q6ZT62-2: 66180 Da (predicted).

Subunit：Interacts with RAC1 (By similarity)

Synonyms

ARHGAP43

Documentation

User Guide for Antibodies (1077 KB)