TRPM2 Antibody
(Synonyms: EREG1, KNP3, LTRPC2, TRPC7, TRPM2, Transient receptor potential cation channel subfamily M member 2, Estrogen-responsive element-associated gene 1 protein, Long transient receptor potential channel 2, Transient receptor potential channel 7, Transient receptor potential melastatin 2, LTrpC-2, LTrpC2, TrpC7)TRPM2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to TRPM2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in TBS (pH7.4), 0.2% BSA, 50% Glycerol and 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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FC
FC: Flow Cytometry
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|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:100-500 |
Product Details
TRPM2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to TRPM2.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 171 kDa
Synthetic peptide of mouse TRPM2 (aa 100-150).
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in TBS (pH7.4), 0.2% BSA, 50% Glycerol and 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
TRPM2 is a Ca2+-permeable, non-selective cation channel activated by temperature, oxidative stress, and NAD+-related metabolites such as ADP-ribose[1]. Mechanistically, TRPM2 connects reactive oxygen species to Ca2+ influx, immune response, apoptosis, and core body temperature regulation[1]. In inflammatory models, TRPM2-mediated Ca2+ influx drives chemokine production in monocytes and aggravates neutrophil infiltration[2]. TRPM2 also links oxidative stress to NLRP3 inflammasome activation, and Trpm2−/− mice resist crystal- or liposome-induced IL-1β-mediated peritonitis[3]. In sensory physiology, TRPM2 is required for non-noxious warmth detection in mice, defining a heat-sensitive role distinct from cold- and menthol-activated TRPM8[4]. For experimental applications, 2-APB rapidly and reversibly inhibits ADP-ribose-, cADPR-, and heat-activated TRPM2, while ACA inhibits H2O2-induced TRPM2-mediated Ca2+ entry in β-cells[5][6].
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Subcellular Localization
Cell membrane; Multi-pass membrane protein, Lysosome. Isoform 1: Cell membrane; Multi-pass membrane protein; Isoform 2: Cell membrane; Multi-pass membrane protein; Isoform 3: Cell membrane; Multi-pass membrane protein
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Expression
Tissue specificity: Highly expressed in brain and peripheral blood cells, such as neutrophils. Also detected in bone marrow, spleen, heart, liver and lung. Isoform 2 is found in neutrophil granulocytes. -
Isoforms & Post-Translational Modification
TRPM2 exists in three isomers: TRPM2-L: 1503 amino acids, molecular weight 171,198 Da (predicted); O94759-2: 1449 amino acids, molecular weight 165,273 Da (predicted); TRPM2-S: 846 amino acids, molecular weight 95,699 Da (predicted).
Protein kinase C (PKC) phosphorylation of TRPM2 at Thr-740 can counteract the effects of cytoplasmic Ca2+ and increase the temperature threshold. -
Subunit
Homotetramer. Isoform 1 can interact with isoform 3. This interaction decreases Ca2+ influx through isoform 1 and suppresses susceptibility to oxidative stress-induced cell death.
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SwissProt ID
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Synonyms
EREG1, KNP3, LTRPC2, TRPC7, TRPM2, Transient receptor potential cation channel subfamily M member 2, Estrogen-responsive element-associated gene 1 protein, Long transient receptor potential channel 2, Transient receptor potential channel 7, Transient receptor potential melastatin 2, LTrpC-2, LTrpC2, TrpC7
Documentation
[1]. Huang Y, et al. Architecture of the TRPM2 channel and its activation mechanism by ADP-ribose and calcium. Nature. 2018 Oct;562(7725):145-149. [Content Brief]
[2]. Yamamoto S, et al. TRPM2-mediated Ca2+influx induces chemokine production in monocytes that aggravates inflammatory neutrophil infiltration. Nat Med. 2008 Jul;14(7):738-47. [Content Brief]
[3]. Zhong Z, et al. TRPM2 links oxidative stress to NLRP3 inflammasome activation. Nat Commun. 2013;4:1611. [Content Brief]
[4]. Tan CH, et al. The TRPM2 ion channel is required for sensitivity to warmth. Nature. 2016 Aug 25;536(7617):460-3. [Content Brief]
[5]. Togashi K, et al. Inhibition of the transient receptor potential cation channel TRPM2 by 2-aminoethoxydiphenyl borate (2-APB). Br J Pharmacol. 2008 Mar;153(6):1324-30. [Content Brief]
[6]. Bari MR, et al. H2O2-induced Ca2+ influx and its inhibition by N-(p-amylcinnamoyl) anthranilic acid in the beta-cells: involvement of TRPM2 channels. J Cell Mol Med. 2009 Sep;13(9B):3260-7. [Content Brief]
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