Art. -Nr.: HY-151970

STING-IN-4

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STING-IN-4 (Compound 1) is a STING inhibitor that inhibits STING expression and hence reducing activation of STING and nuclear factor-κB (NF-κB) signaling. STING-IN-4 shows anti-inflammatory activity and can be used for the research of sepsis.

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Reinheit: 95.87%
CAS. Nr.: 2250374-27-5
Formel: C₃₂H₄₆N₂O₃
Molecular Weight:506.72

Speicherung:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

STING-IN-4 Chemical Structure

CAS. Nr.: 2250374-27-5

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

STING^[1]

In Vitro

STING-IN-4 (Compound 1) (20 μM; 26 h) inhibits NO production induced by LPS in RAW264.7 cells^[1].
STING-IN-4 (2.5-10 μM; 26 h) significantly inhibits the expression of iNOS in RAW264.7 cells^[1].
STING-IN-4 (5 and 50 μM; 12 h) significantly reduces STING degradation at 49, 52, and 55 °C and it may have an ability to interact with STING and enhance thermal stabilization of STING^[1].
STING-IN-4 (2.5-10 μM; 8 h) inhibits LPS-induced activation of STING/IRF3/NF-κB^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

RAW264.7 cells
Concentration:

2.5, 5 and 10 μM
Incubation Time:

Pre-treated for 2 h followed LPS treatment for 6 h or 24 h
Result:

Inhibited the expression of iNOS (24 h). Blocked LPS-induced phosphorylation of TBK1, IRF3, p65, and IκB-α (6 h).

In Vivo

STING-IN-4 (Compound 1) (1-9 mg/kg; i.p.; daily for 3 days) protects against LPS-induced liver injuries in mice and inhibits STING/IRF3/NF-κB activation in septic mice livers^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c mice, LPS-induced acute liver injury model^[1]
Dosage:

1, 3 and 9 mg/kg
Administration:

Intraperitoneal injection, daily for 3 days
Result:

Significantly reduced the hemorrhage severity. Decreased the levels of alanine transaminase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) induced by LPS. Significantly reduced the levels of TNF-α, IL-6, and IFN-β compared with mice treated only with LPS. Markedly reduced the levels of STING, p-TBK, p-IRF3, p-p65, and p-IκB-α.

Chemical Information

CAS. Nr. 2250374-27-5
Appearance Solid
Molecular Weight 506.72
Formel C₃₂H₄₆N₂O₃
Color White to off-white
SMILES

C[C@@]12[C@]3([H])[C@]([C@]4(C([C@@]5([H])[C@@](CC4)(CCC(C)(C5)C)C(O)=O)=CC3)C)(CC[C@@]1([H])[C@@](C)(C6=NC=CN=C6C2)CO)C
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 93.33 mg/mL (184.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9735 mL

9.8674 mL

19.7348 mL

5 mM

0.3947 mL

1.9735 mL

3.9470 mL

10 mM

0.1973 mL

0.9867 mL

1.9735 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 3.5 mg/mL (6.91 mM); Clear solution

This protocol yields a clear solution of ≥ 3.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (35.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 3.5 mg/mL (6.91 mM); Clear solution

This protocol yields a clear solution of ≥ 3.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (35.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Yu T, et al. Design and synthesis of hederagenin derivatives modulating STING/NF-κB signaling for the relief of acute liver injury in septic mice. Eur J Med Chem. 2023 Jan 5;245(Pt 1):114911. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9735 mL	9.8674 mL	19.7348 mL	49.3369 mL
	5 mM	0.3947 mL	1.9735 mL	3.9470 mL	9.8674 mL
	10 mM	0.1973 mL	0.9867 mL	1.9735 mL	4.9337 mL
	15 mM	0.1316 mL	0.6578 mL	1.3157 mL	3.2891 mL
	20 mM	0.0987 mL	0.4934 mL	0.9867 mL	2.4668 mL
	25 mM	0.0789 mL	0.3947 mL	0.7894 mL	1.9735 mL
	30 mM	0.0658 mL	0.3289 mL	0.6578 mL	1.6446 mL
	40 mM	0.0493 mL	0.2467 mL	0.4934 mL	1.2334 mL
	50 mM	0.0395 mL	0.1973 mL	0.3947 mL	0.9867 mL
	60 mM	0.0329 mL	0.1645 mL	0.3289 mL	0.8223 mL
	80 mM	0.0247 mL	0.1233 mL	0.2467 mL	0.6167 mL
	100 mM	0.0197 mL	0.0987 mL	0.1973 mL	0.4934 mL

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STING-IN-4

Immunology/Inflammation Compound Library

NF-κB Signaling Compound Library

Small Molecule Immuno-Oncology Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

STING-IN-4

Immunology/Inflammation Compound Library

NF-κB Signaling Compound Library

Small Molecule Immuno-Oncology Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

STING-IN-4 Related Classifications

HY-151970 Related Classifications