Boc-Leu-Gly-Arg-AMC

Name: Boc-Leu-Gly-Arg-AMC
Brand: MedChemExpress
SKU: HY-P2237
Rating: 4.5 (1 reviews)

Cat. No.: HY-P2237 Purity: 98.35%: FAQs
Data Sheet SDS COA Handling Instructions

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Boc-Leu-Gly-Arg-AMC is a fluorogenic AMC substrate for the convertases. Boc-Leu-Gly-Arg-AMC can be used in enzymatic assays.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Boc-Leu-Gly-Arg-AMC Chemical Structure

CAS No. : 65147-09-3

Size	Price	Stock	Quantity
5 mg	USD 125	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 195	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 660	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1150	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Boc-Leu-Gly-Arg-AMC is a fluorogenic AMC substrate for the convertases. Boc-Leu-Gly-Arg-AMC can be used in enzymatic assays^[1]^[2].

In Vitro

To demonstrate the presence in the abdominal gland of proteolytic enzymes capable of generating Sodefrin, an enzymatic assay was developed using Boc-Leu-Gly-Arg-AMC as synthetic substrate. A crude extract of the abdominal gland hydrolyzed Boc-Leu-Gly-Arg-AMC to liberate 7-amino-4- methylcoumarin, suggesting that enzymes that generate sodefrin from its precursor molecule are present in the gland. The activity in the extract for cleaving Boc-Leu-Gly-Arg-AMC is optimal at pH 9.0 and 45 ℃^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

601.69

Formula

C₂₉H₄₃N₇O₇

CAS No.

65147-09-3

Appearance

Solid

Color

White to off-white

Sequence

Boc-Leu-Gly-Arg-AMC

Sequence Shortening

Boc-LGR-AMC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (166.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6620 mL	8.3099 mL	16.6199 mL
5 mM	0.3324 mL	1.6620 mL	3.3240 mL
10 mM	0.1662 mL	0.8310 mL	1.6620 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.35%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (248 KB) LCMS (261 KB)

Handling Instructions (2659 KB)

References

[1]. Nakada T, et al. Evidence for processing enzymes in the abdominal gland of the newt, Cynops pyrrhogaster, that generate sodefrin from its biosynthetic precursor. Zoolog Sci. 2007 May;24(5):521-4. [Content Brief]

[2]. Yumiko Obayashi, et al. Proteolytic enzymes in coastal surface seawater: Significant activity of endopeptidases and exopeptidases Limnol. Oceanogr., 50(2), 2005, 722-726.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6620 mL	8.3099 mL	16.6199 mL	41.5496 mL
	5 mM	0.3324 mL	1.6620 mL	3.3240 mL	8.3099 mL
	10 mM	0.1662 mL	0.8310 mL	1.6620 mL	4.1550 mL
	15 mM	0.1108 mL	0.5540 mL	1.1080 mL	2.7700 mL
	20 mM	0.0831 mL	0.4155 mL	0.8310 mL	2.0775 mL
	25 mM	0.0665 mL	0.3324 mL	0.6648 mL	1.6620 mL
	30 mM	0.0554 mL	0.2770 mL	0.5540 mL	1.3850 mL
	40 mM	0.0415 mL	0.2077 mL	0.4155 mL	1.0387 mL
	50 mM	0.0332 mL	0.1662 mL	0.3324 mL	0.8310 mL
	60 mM	0.0277 mL	0.1385 mL	0.2770 mL	0.6925 mL
	80 mM	0.0208 mL	0.1039 mL	0.2077 mL	0.5194 mL
	100 mM	0.0166 mL	0.0831 mL	0.1662 mL	0.4155 mL