1. MAPK/ERK Pathway
    Stem Cell/Wnt
    Metabolic Enzyme/Protease
  2. p38 MAPK
    MEK
    ERK
    Endogenous Metabolite
  3. C16-PAF

C16-PAF (Synonyms: PAF (C16))

Cat. No.: HY-108635 Purity: ≥98.0%
Handling Instructions

C16-PAF (PAF (C16)), a phospholipid mediator, is a platelet-activating factor and ligand for PAF G-protein-coupled receptor (PAFR). C16-PAF exhibits anti-apoptotic effect and inhibits caspase-dependent death by activating the PAFR. C16-PAF is a potent MAPK and MEK/ERK activator. C16-PAF induces increased vascular permeability.

For research use only. We do not sell to patients.

C16-PAF Chemical Structure

C16-PAF Chemical Structure

CAS No. : 74389-68-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 403 In-stock
Estimated Time of Arrival: December 31
5 mg USD 350 In-stock
Estimated Time of Arrival: December 31
10 mg USD 550 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

View All p38 MAPK Isoform Specific Products:

View All MEK Isoform Specific Products:

View All ERK Isoform Specific Products:

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

C16-PAF (PAF (C16)), a phospholipid mediator, is a platelet-activating factor and ligand for PAF G-protein-coupled receptor (PAFR). C16-PAF exhibits anti-apoptotic effect and inhibits caspase-dependent death by activating the PAFR. C16-PAF is a potent MAPK and MEK/ERK activator. C16-PAF induces increased vascular permeability[1][2][3][4][5].

IC50 & Target[1][4][5]

Human Endogenous Metabolite

 

ERK

 

MEK

 

In Vitro

C16-PAF (PAF (C16); 0.5-1.5 μM; for 24 hours) elicits significant concentration-dependent neuronal loss in PAFR−/− but not PAFR+/+ cultures. C16-PAF (1 μM) elicits neuronal death in PAFR−/− cells infected with EGFP alone[1].
C16-PAF (1 μM; for 24 hours) activates caspase 7 but not caspase 3 in PAFR−/− neurons[1].
C16-PAF is synthesized by two distinct pathways; the remodeling pathway and the de novo synthesis pathway. C16-PAF acts by binding to a unique G-protein-coupled seven transmembrane receptor[2][3].
C16-PAF (1-25 μg/ml; 6, 12, 24 h) inhibits M. smegmatis and M. bovis BCG growth in a time-dependent manner[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Cerebellar granule neurons (CGNs) from PAFR−/− and PAFR+/+ mice
Concentration: 0.5-1.5 μM
Incubation Time: 24 hours
Result: Elicited significant concentration-dependent neuronal loss in PAFR−/− but not PAFR+/+ cultures in serum-free media.

Western Blot Analysis[1]

Cell Line: CGNs
Concentration: 1 μM
Incubation Time: 24 hours
Result: Activated caspase 7 but not caspase 3 in PAFR−/− neurons.
Molecular Weight

523.68

Formula

C₂₆H₅₄NO₇P

CAS No.

74389-68-7

SMILES

CCCCCCCCCCCCCCCCOC[[email protected]@H](OC(C)=O)COP(OCC[N+](C)(C)C)([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (95.48 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9096 mL 9.5478 mL 19.0956 mL
5 mM 0.3819 mL 1.9096 mL 3.8191 mL
10 mM 0.1910 mL 0.9548 mL 1.9096 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (4.77 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (4.77 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: ≥98.0%

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

C16-PAFPAF (C16)p38 MAPKMEKERKEndogenous MetaboliteMitogen-activated protein kinase kinaseMAPKKMAP2KExtracellular signal regulated kinasesphospholipidmediatorplatelet-activatingfactorPAFPAFRapoptoticvascularpermeabilityInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
C16-PAF
Cat. No.:
HY-108635
Quantity:
MCE Japan Authorized Agent: