The crystal structure of the eukaryotic 40S ribosomal subunit in complex with eIF1 and eIF1A

  • Nat Struct Mol Biol. 2013 Aug;20(8):1015-7. doi: 10.1038/nsmb.2622.
Melanie Weisser  1 ,  Felix Voigts-Hoffmann ,  Julius Rabl ,  Marc Leibundgut ,  Nenad Ban
Affiliations
  • 1. Institute of Molecular Biology and Biophysics, Swiss Federal Institute of Technology (ETH Zürich), Zürich, Switzerland.
PMID: 23851459 DOI: 10.1038/nsmb.2622
Abstract

Eukaryotic translation initiation factors (eIFs) 1A and 1 are central players in the complex process of start-codon recognition. To improve mechanistic understanding of this process, we determined the crystal structure of the 40S ribosomal subunit in complex with eIF1A and eIF1 from Tetrahymena thermophila at a resolution of 3.7 Å. It reveals the positions of the two factors on the 40S and the conformational changes that accompany their binding.