Cat. No.: HY-W018643

Ferulic acid methyl ester

(Synonyms: Methyl ferulate)

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Ferulic acid methyl ester (Methyl ferulate) is a derivative of ferulic acid, isolated from Stemona tuberosa, with anti-inflammatory and antioxidant properties. Ferulic acid methyl ester is a cell membrane and brain permeable compound, shows free radical scavenging ability, used in the research of neurodegenerative disorders. Ferulic acid methyl ester inhibits COX-2 expression, blocks p-p38 and p-JNK in primary bone marrow derived-macrophages.

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Pureté: 99.92%
CAS No.: 2309-07-1
Formule: C₁₁H₁₂O₄
Masse moléculaire:208.21

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Ferulic acid methyl ester Chemical Structure

CAS No.: 2309-07-1

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Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (1)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Ferulic acid methyl ester (Methyl ferulate) is a derivative of ferulic acid, isolated from Stemona tuberosa, with anti-inflammatory and antioxidant properties^[1]^[2]. Ferulic acid methyl ester is a cell membrane and brain permeable compound, shows free radical scavenging ability, used in the research of neurodegenerative disorders^[1]. Ferulic acid methyl ester inhibits COX-2 expression, blocks p-p38 and p-JNK in primary bone marrow derived-macrophages^[2].

IC₅₀ & Target^[2]

p38

Voir tous les produits spécifiques à Isoform p38 MAPK :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>20 μg/mL Compound: 17	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 29975532]
A549	IC₅₀	>50 μM Compound: 1a	Cytotoxicity against human A549 cells after 72 hrs by alamar blue assay Cytotoxicity against human A549 cells after 72 hrs by alamar blue assay	[PMID: 22954735]
Calu-1	IC₅₀	>50 μM Compound: 1a	Cytotoxicity against human Calu1 cells after 72 hrs by alamar blue assay Cytotoxicity against human Calu1 cells after 72 hrs by alamar blue assay	[PMID: 22954735]
HeLa	IC₅₀	5.55 μM Compound: 1a	Cytotoxicity against human HeLa cells after 72 hrs by alamar blue assay Cytotoxicity against human HeLa cells after 72 hrs by alamar blue assay	[PMID: 22954735]
HepG2	IC₅₀	>20 μg/mL Compound: 17	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 29975532]
HOP-62	IC₅₀	>50 μM Compound: 1a	Cytotoxicity against human HOP62 cells after 72 hrs by alamar blue assay Cytotoxicity against human HOP62 cells after 72 hrs by alamar blue assay	[PMID: 22954735]
KB	IC₅₀	>200 μM Compound: 1c	Antitumor activity against KB cells by MTT assay Antitumor activity against KB cells by MTT assay	[PMID: 17387015]
LOX IMVI	IC₅₀	>50 μM Compound: 1a	Cytotoxicity against human LOXIMVI cells after 72 hrs by alamar blue assay Cytotoxicity against human LOXIMVI cells after 72 hrs by alamar blue assay	[PMID: 22954735]
M14	IC₅₀	>50 μM Compound: 1a	Cytotoxicity against human M14 cells after 72 hrs by alamar blue assay Cytotoxicity against human M14 cells after 72 hrs by alamar blue assay	[PMID: 22954735]
MDA-MB-231	IC₅₀	>20 μg/mL Compound: 17	Cytotoxicity against human MDA-MB-231 cells by MTT assay Cytotoxicity against human MDA-MB-231 cells by MTT assay	[PMID: 29975532]
NCI-H1299	IC₅₀	>50 μM Compound: 1a	Cytotoxicity against human NCI-H1299 cells after 72 hrs by alamar blue assay Cytotoxicity against human NCI-H1299 cells after 72 hrs by alamar blue assay	[PMID: 22954735]
NCI-H157	IC₅₀	>50 μM Compound: 1a	Cytotoxicity against human NCI-H157 cells after 72 hrs by alamar blue assay Cytotoxicity against human NCI-H157 cells after 72 hrs by alamar blue assay	[PMID: 22954735]
NCI-H1792	IC₅₀	>50 μM Compound: 1a	Cytotoxicity against human NCI-H1792 cells after 72 hrs by alamar blue assay Cytotoxicity against human NCI-H1792 cells after 72 hrs by alamar blue assay	[PMID: 22954735]
NCI-H460	IC₅₀	>50 μM Compound: 1a	Cytotoxicity against human H460 cells after 72 hrs by alamar blue assay Cytotoxicity against human H460 cells after 72 hrs by alamar blue assay	[PMID: 22954735]

In Vitro

Ferulic acid methyl ester (25 µg/mL) has no cytotoxic effects on BMDMs after treatment for 6 h, 18 h, 48 h^[2].
Ferulic acid methyl ester (Methyl ferulate; 5, 10, 25 µg/mL) suppresses TNFα, IL6, IFNγ but not IL10, inhibits NO generation at 10 and 25 µg/mL, in primary bone marrow derived-macrophages (BMDMs)^[2].
Ferulic acid methyl ester (25 µg/mL) inhibits COX-2 expression, blocks p-p38 and p-JNK^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

Primary bone marrow derived-macrophages (BMDMs)
Concentration:

25 µg/mL
Incubation Time:

6 h, 18 h, 48 h
Result:

Showed no cytotoxic effects on BMDMs.

Western Blot Analysis^[2]

Cell Line:

RAW 246.7 cells
Concentration:

10 µg/mL and 25 µg/mL
Incubation Time:

For 1 h before stimulation with LPS
Result:

Significantly suppressed COX-2 expression at 25 µg/mL.

Chemical Information

CAS No. 2309-07-1
Appearance Solid
Masse moléculaire 208.21
Formule C₁₁H₁₂O₄
Color Off-white to pink
SMILES

O=C(/C=C/C1=CC=C(C(OC)=C1)O)OC
Synonyms

Methyl ferulate
Structure Classification
- Phenylpropanoids
- Simple Phenylpropanols
- Phenols
- Monophenols
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (1)

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Journal Impact Factor

Fish Shellfish Immunol

Emodin enhances host antiviral immunity against Micropterus salmoides rhabdovirus by activating RLR signaling in largemouth bass. [Abstract]

2025 Aug 6:166:110633. PMID: 40769268

Solvant et solubilité

In Vitro:

DMSO : ≥ 100 mg/mL (480.28 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 2 mg/mL (9.61 mM; Need ultrasonic)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.8028 mL

24.0142 mL

48.0284 mL

5 mM

0.9606 mL

4.8028 mL

9.6057 mL

10 mM

0.4803 mL

2.4014 mL

4.8028 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (12.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (12.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (12.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (274 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Sultana R. Ferulic acid ethyl ester as a potential therapy in neurodegenerative disorders. Biochim Biophys Acta. 2012 May;1822(5):748-52. [Content Brief]

[2]. Phuong NT, et al. Anti-inflammatory activity of methyl ferulate isolated from Stemona tuberosa Lour. Asian Pac J Trop Med. 2014 Sep;7S1:S327-31. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	4.8028 mL	24.0142 mL	48.0284 mL	120.0711 mL
H₂O / DMSO	5 mM	0.9606 mL	4.8028 mL	9.6057 mL	24.0142 mL
DMSO	10 mM	0.4803 mL	2.4014 mL	4.8028 mL	12.0071 mL
	15 mM	0.3202 mL	1.6009 mL	3.2019 mL	8.0047 mL
	20 mM	0.2401 mL	1.2007 mL	2.4014 mL	6.0036 mL
	25 mM	0.1921 mL	0.9606 mL	1.9211 mL	4.8028 mL
	30 mM	0.1601 mL	0.8005 mL	1.6009 mL	4.0024 mL
	40 mM	0.1201 mL	0.6004 mL	1.2007 mL	3.0018 mL
	50 mM	0.0961 mL	0.4803 mL	0.9606 mL	2.4014 mL
	60 mM	0.0800 mL	0.4002 mL	0.8005 mL	2.0012 mL
	80 mM	0.0600 mL	0.3002 mL	0.6004 mL	1.5009 mL
	100 mM	0.0480 mL	0.2401 mL	0.4803 mL	1.2007 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Ferulic acid methyl ester

1 Cited Publications

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Ferulic acid methyl ester

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Publications Citing Use of MedChemExpress (MCE) Ferulic acid methyl ester

Voir tous les produits spécifiques à Isoform p38 MAPK

Activité biologique

Chemical Information

Publications (1)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Ferulic acid methyl ester Related Classifications

HY-W018643 Related Classifications