Methyl p-coumarate
Based on 1 Customer Validation
Methyl p-coumarate (Methyl 4-hydroxycinnamate), an orally active esterified derivative of p-Coumaric acid (pCA), can be isolated from the flower of Trixis michuacana var longifolia. Methyl p-coumarate could inhibit the melanin formation in B16 mouse melanoma cells. Methyl p-coumarate also has strong in vitro inhibitory effect on A. alternata and other pathogens.
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- Purity : 99.80%
- CAS No.: 3943-97-3
- 화학식: C10H10O3
- 분자량:178.18
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| 3T3-L1 | IC50 |
118.8 μM
Compound: 4
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Antiadipogenic activity in mouse 3T3L1 cells assessed as differentiation of preadipocytes to adipocytes after 8 days by spectrophotometry
Antiadipogenic activity in mouse 3T3L1 cells assessed as differentiation of preadipocytes to adipocytes after 8 days by spectrophotometry
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[PMID: 23628332] |
| A549 | IC50 |
322.92 μM
Compound: pcm
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Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30615450] |
| HaCaT | IC50 |
311.22 μM
Compound: pcm
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Cytotoxicity against human HaCaT cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human HaCaT cells assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30615450] |
| HeLa | IC50 |
>150 μM
Compound: pcm
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Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30615450] |
| HT-1080 | ED50 |
>100 μM
Compound: 16
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Antiproliferative activity against human HT1080 cells by MTT assay
Antiproliferative activity against human HT1080 cells by MTT assay
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[PMID: 11277741] |
| KB | IC50 |
123.1 μM
Compound: 1b
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Antitumor activity against KB cells by MTT assay
Antitumor activity against KB cells by MTT assay
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[PMID: 17387015] |
| MCF7 | IC50 |
>400 μM
Compound: pcm
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Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30615450] |
| NCI-H460 | IC50 |
120.01 μM
Compound: pcm
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Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30615450] |
| NCI-H661 | IC50 |
289.21 μM
Compound: pcm
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Cytotoxicity against human NCI-H661 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human NCI-H661 cells assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30615450] |
| RAW264.7 | GI50 |
609 μM
Compound: 2a
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Cytotoxicity against mouse RAW264.7 cells assessed as growth inhibition after 48 hrs by trypan blue assay
Cytotoxicity against mouse RAW264.7 cells assessed as growth inhibition after 48 hrs by trypan blue assay
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10.1039/C3MD00251A |
| SiHa | IC50 |
>150 μM
Compound: pcm
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Cytotoxicity against human SiHa cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human SiHa cells assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30615450] |
In Vitro
Methyl p-coumarate (10 μM) inhibits melanin formation in B16 mouse melanoma cells[1].
Methyl p-coumarate (200 μg/mL) shows antifungal activity against A. alternata, and inhibits Alternaria rot in jujube fruit[2].
Methyl p-coumarate (0-100 μM, 1 h) shows anti-inflammatory activity on Phorbol 12-myristate 13-acetate (HY-18739) (10 nM, 18 h)-stimulated A549 cells by suppressing the secretion of IL-6, IL-8, MCP-1, and ICAM-1[3].
Methyl p-coumarate (0-100 μM, 1 h) inhibits activation of NF-κB/AP-1 in Phorbol 12-myristate 13-acetate (HY-18739) (10 nM, 18 h)-stimulated A549 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
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Cell Line:Phorbol 12-myristate 13-acetate (HY-18739) (10 nM, 1 h)-stimulated A549 cells
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Concentration:0-100 μM
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Incubation Time:1 h
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Result:Inhibited PMA-stimulated phosphorylation of IκBα and p65, as well as c-Jun/c-Fos phosphorylation and c-Jun/c-Fos nuclear translocation.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Ovalbumins (HY-W250978)-induced mice with allergic asthma[3]
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Dosage:5 mg/kg
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Administration:p.o.
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Result:Inhibited the secretion of Th2 cytokines, MCP-1, and IgE.
Inhibited eosinophil/macrophage influx.
Inhibited immune cell influx and mucus secretion.
Chemical Information
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CAS No. 3943-97-3
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Appearance Solid
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분자량 178.18
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화학식 C10H10O3
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Color Off-white to light yellow
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SMILES
O=C(OC)/C=C/C1=CC=C(O)C=C1
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Synonyms
Methyl 4-hydroxycinnamate
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Structure Classification
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Initial Source
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
In Vitro:
DMSO : 100 mg/mL (561.23 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (14.03 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.03 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (273 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Kubo I, et, al. Methyl p-coumarate, a melanin formation inhibitor in B16 mouse melanoma cells. Bioorg Med Chem. 2004 Oct 15;12(20):5349-54. [Content Brief]
[2]. Yuan S, et, al. Defense Responses, Induced by p-Coumaric Acid and Methyl p-Coumarate, of Jujube ( Ziziphus jujuba Mill.) Fruit against Black Spot Rot Caused by Alternaria alternate. J Agric Food Chem. 2019 Mar 13;67(10):2801-2810. [Content Brief]
[3]. Park JW, et al. Methyl P-Coumarate Ameliorates the Inflammatory Response in Activated-Airway Epithelial Cells and Mice with Allergic Asthma. Int J Mol Sci. 2022 Nov 28;23(23):14909. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.6123 mL | 28.0615 mL | 56.1230 mL | 140.3076 mL |
| 5 mM | 1.1225 mL | 5.6123 mL | 11.2246 mL | 28.0615 mL | |
| 10 mM | 0.5612 mL | 2.8062 mL | 5.6123 mL | 14.0308 mL | |
| 15 mM | 0.3742 mL | 1.8708 mL | 3.7415 mL | 9.3538 mL | |
| 20 mM | 0.2806 mL | 1.4031 mL | 2.8062 mL | 7.0154 mL | |
| 25 mM | 0.2245 mL | 1.1225 mL | 2.2449 mL | 5.6123 mL | |
| 30 mM | 0.1871 mL | 0.9354 mL | 1.8708 mL | 4.6769 mL | |
| 40 mM | 0.1403 mL | 0.7015 mL | 1.4031 mL | 3.5077 mL | |
| 50 mM | 0.1122 mL | 0.5612 mL | 1.1225 mL | 2.8062 mL | |
| 60 mM | 0.0935 mL | 0.4677 mL | 0.9354 mL | 2.3385 mL | |
| 80 mM | 0.0702 mL | 0.3508 mL | 0.7015 mL | 1.7538 mL | |
| 100 mM | 0.0561 mL | 0.2806 mL | 0.5612 mL | 1.4031 mL |
Methyl p-coumarate Related Classifications
HY-N1434 Related Classifications
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