(E)-Methyl 4-coumarate
Based on 1 Customer Validation
(E)-Methyl 4-coumarate (Methyl 4-hydroxycinnamate) is a phenolic compound and derivative of Cinnamic acid (HY-N0610A). (E)-Methyl 4-coumarate can be found in several plants, such as the leaves of Allium cepa and Morinda citrifolia L. (E)-Methyl 4-coumarate, when combined with Carnosic acid (HY-N0644), induces Apoptosis. (E)-Methyl 4-coumarate inhibits GSK3β activity and modulates inflammatory cytokine levels (increasing IL-10 and decreasing IL-4). (E)-Methyl 4-coumarate combined with Carnosic acid exhibits anticancer effects against acute myeloid leukemia. (E)-Methyl 4-coumarate ameliorates Plasmodium berghei NK65 infection.
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- Pureza: 99.90%
- No. CAS: 19367-38-5
- Fòrmula: C10H10O3
- Peso molecular:178.19
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Descripciòn
IC50 & Target
[2]|
GSK-3β |
IL-10 |
IL-4 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| 3T3-L1 | IC50 |
118.8 μM
Compound: 4
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Antiadipogenic activity in mouse 3T3L1 cells assessed as differentiation of preadipocytes to adipocytes after 8 days by spectrophotometry
Antiadipogenic activity in mouse 3T3L1 cells assessed as differentiation of preadipocytes to adipocytes after 8 days by spectrophotometry
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[PMID: 23628332] |
| A549 | IC50 |
322.92 μM
Compound: pcm
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Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30615450] |
| HaCaT | IC50 |
311.22 μM
Compound: pcm
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Cytotoxicity against human HaCaT cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human HaCaT cells assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30615450] |
| HeLa | IC50 |
>150 μM
Compound: pcm
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Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30615450] |
| HT-1080 | ED50 |
>100 μM
Compound: 16
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Antiproliferative activity against human HT1080 cells by MTT assay
Antiproliferative activity against human HT1080 cells by MTT assay
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[PMID: 11277741] |
| KB | IC50 |
123.1 μM
Compound: 1b
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Antitumor activity against KB cells by MTT assay
Antitumor activity against KB cells by MTT assay
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[PMID: 17387015] |
| MCF7 | IC50 |
>400 μM
Compound: pcm
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Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30615450] |
| NCI-H460 | IC50 |
120.01 μM
Compound: pcm
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Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30615450] |
| NCI-H661 | IC50 |
289.21 μM
Compound: pcm
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Cytotoxicity against human NCI-H661 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human NCI-H661 cells assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30615450] |
| RAW264.7 | GI50 |
609 μM
Compound: 2a
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Cytotoxicity against mouse RAW264.7 cells assessed as growth inhibition after 48 hrs by trypan blue assay
Cytotoxicity against mouse RAW264.7 cells assessed as growth inhibition after 48 hrs by trypan blue assay
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10.1039/C3MD00251A |
| SiHa | IC50 |
>150 μM
Compound: pcm
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Cytotoxicity against human SiHa cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human SiHa cells assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30615450] |
In Vitro
(E)-Methyl 4-coumarate (5 µM; 72 h) synergizes strongly with Carnosic acid (HY-N0644) to reduce the number of viable cells in HL60, KG-1a, and U937 acute myeloid leukemia (AML) cell lines[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Animal Model: ICR mice infected with Plasmodium berghei NK65[2]
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Dosage:10 mg/kg, 30 mg/kg, 60 mg/kg
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Administration:Intraperitoneal injection (i.p), consecutive administration, 4-day cycle
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Result:Resulted in average parasitaemia suppression rates of 62.45 % (10 mg/kg), 68.44% (30 mg/kg), and 51.84% (60 mg/kg) respectively on day 4 post-infection.
Showed median survival times of 15 days, 19 days, and 17 days at doses of 10 mg/kg, 30 mg/kg, and 60 mg/kg, respectively.
Reduced serum pro-inflammatory cytokines TNF-α and IFN-γ to 0.1-fold and 0.4-fold of the control group respectively, increased anti-inflammatory cytokine IL-10 to 3.0-fold of the control group, and decreased IL-4 to 0.6-fold of the control group.
Increased the level of pGSK3β (Ser9) in the liver of infected mice by 7.0-fold compared to the control group, indicating inhibition of GSK3β activity.
Chemical Information
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No. CAS 19367-38-5
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Appearance Solid
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Peso molecular 178.19
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Fòrmula C10H10O3
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Color White to off-white
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SMILES
O=C(OC)/C=C/C1=CC=C(O)C=C1
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Synonyms
Methyl trans-p-coumarate
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (561.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.03 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.03 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (273 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.6120 mL | 28.0599 mL | 56.1199 mL | 140.2997 mL |
| 5 mM | 1.1224 mL | 5.6120 mL | 11.2240 mL | 28.0599 mL | |
| 10 mM | 0.5612 mL | 2.8060 mL | 5.6120 mL | 14.0300 mL | |
| 15 mM | 0.3741 mL | 1.8707 mL | 3.7413 mL | 9.3533 mL | |
| 20 mM | 0.2806 mL | 1.4030 mL | 2.8060 mL | 7.0150 mL | |
| 25 mM | 0.2245 mL | 1.1224 mL | 2.2448 mL | 5.6120 mL | |
| 30 mM | 0.1871 mL | 0.9353 mL | 1.8707 mL | 4.6767 mL | |
| 40 mM | 0.1403 mL | 0.7015 mL | 1.4030 mL | 3.5075 mL | |
| 50 mM | 0.1122 mL | 0.5612 mL | 1.1224 mL | 2.8060 mL | |
| 60 mM | 0.0935 mL | 0.4677 mL | 0.9353 mL | 2.3383 mL | |
| 80 mM | 0.0701 mL | 0.3507 mL | 0.7015 mL | 1.7537 mL | |
| 100 mM | 0.0561 mL | 0.2806 mL | 0.5612 mL | 1.4030 mL |
(E)-Methyl 4-coumarate Related Classifications
HY-N2492 Related Classifications
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