CD33 splicing modulator 1

Name: CD33 splicing modulator 1
Brand: MedChemExpress
SKU: HY-144399
Rating: 4.5 (0 reviews)

製品番号: HY-144399 純度: 99.05%: Data Sheet SDS COA 取扱説明書
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CD33 splicing modulator 1 (Compound 1) is a CD33 splicing modulator. CD33/Siglec 3 is a myeloid lineage cell surface receptor that is known to regulate microglia activity. CD33 splicing modulator 1 increases exon 2 skipping in cellular mRNA pools. CD33 splicing modulator 1 has the potential for the research of neurodegenerative disease including Alzheimer's disease.

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CD33 splicing modulator 1 構造式

CAS 番号 : 2762547-05-5

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 352	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 352	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$320	在庫あり	Estimated Time of Arrival: December 31
10 mg	$520	在庫あり	Estimated Time of Arrival: December 31
25 mg	$1040	在庫あり	Estimated Time of Arrival: December 31
50 mg	$1650	在庫あり	Estimated Time of Arrival: December 31
100 mg	$2640	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

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Based on 0 publication(s) in Google Scholar

Other Forms of CD33 splicing modulator 1:

CD33 splicing modulator 1 hydrochloride お問い合わせ

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製品説明

Cellular Effect

Cell Line	Type	Value	Description	References
THP-1	EC₅₀	2.1 μM Compound: 1	Increase in CD33 level in human THP-1 cell surface incubated for 48 to 72 hrs by high content imaging assay Increase in CD33 level in human THP-1 cell surface incubated for 48 to 72 hrs by high content imaging assay	[PMID: 35059124]
THP-1	EC₅₀	> 10 μM Compound: 1	Cytotoxicity against human THP-1 cells assessed as reduction in cell viability by DAPI staining based assay Cytotoxicity against human THP-1 cells assessed as reduction in cell viability by DAPI staining based assay	[PMID: 35059124]
THP-1	EC₅₀	> 10 μM Compound: 1	Increase in CD11c level in human THP-1 cell surface incubated for 48 to 72 hrs by high content imaging assay Increase in CD11c level in human THP-1 cell surface incubated for 48 to 72 hrs by high content imaging assay	[PMID: 35059124]

分子量

444.50

分子式

C₂₅H₂₅FN₆O

CAS 番号

2762547-05-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

CC1=CN2N=CC(C3=CC=C(C=C3F)C(N([C@@H]4CCCNC4)C5=C(C=CC=N5)C)=O)=C2N=C1

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (224.97 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2497 mL	11.2486 mL	22.4972 mL
5 mM	0.4499 mL	2.2497 mL	4.4994 mL
10 mM	0.2250 mL	1.1249 mL	2.2497 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 5 mg/mL (11.25 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 5 mg/mL (11.25 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 5 mg/mL (11.25 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.05%

Select Batch:

データシート (278 KB) SDS (251 KB)

COA (259 KB) HNMR (148 KB) RP-HPLC (229 KB) MS (229 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Chappie TA, et al. Discovery of Small-Molecule CD33 Pre-mRNA Splicing Modulators. ACS Med Chem Lett. 2022;13(1):55-62. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2497 mL	11.2486 mL	22.4972 mL	56.2430 mL
	5 mM	0.4499 mL	2.2497 mL	4.4994 mL	11.2486 mL
	10 mM	0.2250 mL	1.1249 mL	2.2497 mL	5.6243 mL
	15 mM	0.1500 mL	0.7499 mL	1.4998 mL	3.7495 mL
	20 mM	0.1125 mL	0.5624 mL	1.1249 mL	2.8121 mL
	25 mM	0.0900 mL	0.4499 mL	0.8999 mL	2.2497 mL
	30 mM	0.0750 mL	0.3750 mL	0.7499 mL	1.8748 mL
	40 mM	0.0562 mL	0.2812 mL	0.5624 mL	1.4061 mL
	50 mM	0.0450 mL	0.2250 mL	0.4499 mL	1.1249 mL
	60 mM	0.0375 mL	0.1875 mL	0.3750 mL	0.9374 mL
	80 mM	0.0281 mL	0.1406 mL	0.2812 mL	0.7030 mL
	100 mM	0.0225 mL	0.1125 mL	0.2250 mL	0.5624 mL