1. Cell Cycle/DNA Damage
  2. CDK
  3. CDK5 inhibitor 20-223

CDK5 inhibitor 20-223 

Cat. No.: HY-123772 Purity: 99.64%
Handling Instructions

CDK5 inhibitor 20-223 is a potent CDK2 and CDK5 inhibitor with IC50s of 6.0 and 8.8 nM, respectively. CDK5 inhibitor 20-223 is an effective anti-colorectal cancer (CRC) agent.

For research use only. We do not sell to patients.

CDK5 inhibitor 20-223 Chemical Structure

CDK5 inhibitor 20-223 Chemical Structure

CAS No. : 865317-30-2

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Solution
10 mM * 1 mL in DMSO USD 220 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 220 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 200 In-stock
Estimated Time of Arrival: December 31
10 mg USD 350 In-stock
Estimated Time of Arrival: December 31
25 mg USD 650 In-stock
Estimated Time of Arrival: December 31
50 mg USD 950 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

CDK5 inhibitor 20-223 is a potent CDK2 and CDK5 inhibitor with IC50s of 6.0 and 8.8 nM, respectively. CDK5 inhibitor 20-223 is an effective anti-colorectal cancer (CRC) agent[1].

IC50 & Target[1]

CDK2

6.0 nM (IC50)

CDK5

8.8 nM (IC50)

In Vitro

CDK5 inhibitor 20-223 (10 nM-10 μM; 72 hours) potently inhibits cell growth in a panel of colorectal cancer (CRC) cell lines[1].
CDK5 inhibitor 20-223 (0.3125-20 μM; 6 hours) induces a dose-dependent decrease in pRB (S807/811) and pFAK (S732) levels in each of the three CRC cell lines[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: CRC cell lines SW620, DLD1, HT29, HCT116, FET, CBS, and GEO cells
Concentration: 10 μM, 1 μM, 100 nM, 10 nM
Incubation Time: 72 hours
Result: Reduced cell growth. IC50s of 168±20, 480±41, 360±72, 763±92, 117±49, 568±49, 79±31 nM for SW620, DLD1, HT29, HCT116, FET, CBS, and GEO cells.

Western Blot Analysis[1]

Cell Line: CRC cell lines GEO, HCT116 and HT29
Concentration: 20, 10, 5, 2.5, 1.25, 0.625, 0.3125 μM
Incubation Time: 6 hours
Result: Did not affect the total levels of CDK2/5, and the levels of total FAK or total Retinoblastoma protein (Rb).
Induced a dose-dependent decrease in pRB (S807/811) and pFAK (S732) levels.
In Vivo

CDK5 inhibitor 20-223 (8mg/kg; subcutaneously; for 14 injections) shows anti-tumor activity in human CRC xenograft tumors in nude mice[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Athymic nude mice[1]
Dosage: 8 mg/kg
Administration: Injections were given subcutaneously daily for the first week and every other day for two more weeks for a total of 14 injections.
Result: Reduced tumor growth and tumor weight in vivo.
Molecular Weight

305.37

Formula

C₁₉H₁₉N₃O

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (327.47 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2747 mL 16.3736 mL 32.7472 mL
5 mM 0.6549 mL 3.2747 mL 6.5494 mL
10 mM 0.3275 mL 1.6374 mL 3.2747 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 5 mg/mL (16.37 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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CDK5 inhibitor 20-223
Cat. No.:
HY-123772
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