Art. -Nr.: HY-154986

MARK4 inhibitor 2

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MARK4 inhibitor 2 is an inhibitor of microtubule affinity-regulating kinase 4 (MARK4) with an K_m of 6.3×10 ⁷ and an IC₅₀ of 0.82 μM. MARK4 inhibitor 2 inhibits the growth of human cells and can be used for cancer research.

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Reinheit: 99.60%
Formel: C₃₄H₃₀N₄O₃
Molecular Weight:542.63

Speicherung:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

MARK4 inhibitor 2 Chemical Structure

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

IC50: 0.82 μM (MARK4)^[1] K_m:6.3×10 ⁷^[1]

Alle AMPK Isoform-spezifische Produkte anzeigen :

In Vitro

MARK4 inhibitor 2 (Compound 23a) (0-20 μM; 24 hour) inhibits the growth of HeLa, U87MG, MDA-MB-435, U251 and HGFs cells with EC₅₀s ranging from 1.76 μM to 7.98 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

HeLa, U87MG, MDA-MB-435, U251, HGFs cells
Concentration:

0-20 μM
Incubation Time:

24 hours
Result:

Inhibited the growth of human cells with EC₅₀ s of 2.13, 4.13, 1.76, 7.98, 3.55 μM for HeLa, U87MG, MDA-MB-435, U251, HGFs cells, respectively.
Decreased the proliferation of human cancer cells in the low micromolar range; also affects the non-cancerous HGF cells.

Chemical Information

Appearance Solid
Molecular Weight 542.63
Formel C₃₄H₃₀N₄O₃
Color Light yellow to yellow
SMILES

O=C(NC1=CC=C(C)C=C1)[C@@H](NC(CN(C2=C3C=CC=C2)C4=CC=C(C)C=C4C3=O)=O)CC5=CNC6=C5C=CC=C6
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (184.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.8429 mL

9.2144 mL

18.4288 mL

5 mM

0.3686 mL

1.8429 mL

3.6858 mL

10 mM

0.1843 mL

0.9214 mL

1.8429 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: 2.5 mg/mL (4.61 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Maria Voura, et.al. Synthesis, Structural Modification, and Bioactivity Evaluation of Substituted Acridones as Potent Microtubule Affinity-Regulating Kinase 4 InhibitorsACS Pharmacology & Translational Science 2023 6 (7), 1052-1074.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.8429 mL	9.2144 mL	18.4288 mL	46.0719 mL
	5 mM	0.3686 mL	1.8429 mL	3.6858 mL	9.2144 mL
	10 mM	0.1843 mL	0.9214 mL	1.8429 mL	4.6072 mL
	15 mM	0.1229 mL	0.6143 mL	1.2286 mL	3.0715 mL
	20 mM	0.0921 mL	0.4607 mL	0.9214 mL	2.3036 mL
	25 mM	0.0737 mL	0.3686 mL	0.7372 mL	1.8429 mL
	30 mM	0.0614 mL	0.3071 mL	0.6143 mL	1.5357 mL
	40 mM	0.0461 mL	0.2304 mL	0.4607 mL	1.1518 mL
	50 mM	0.0369 mL	0.1843 mL	0.3686 mL	0.9214 mL
	60 mM	0.0307 mL	0.1536 mL	0.3071 mL	0.7679 mL
	80 mM	0.0230 mL	0.1152 mL	0.2304 mL	0.5759 mL
	100 mM	0.0184 mL	0.0921 mL	0.1843 mL	0.4607 mL

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MARK4 inhibitor 2

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Radioprotector Library

Bioactive Compound Library Max

Anti-Aging Compound Library Mini

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Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Lactic Acid Metabolic Compound Library

High-Throughput Bioactive Compound Library

Alle AMPK Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

MARK4 inhibitor 2

Cell Cycle/DNA Damage Compound Library

Epigenetics Compound Library

PI3K/Akt/mTOR Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Oxygen Sensing Compound Library

Glycolysis Compound Library

Cytoskeleton Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Lipid Metabolism Compound Library

Glucose Metabolism Compound Library

Pain-Related Compound Library

Radioprotector Library

Bioactive Compound Library Max

Anti-Aging Compound Library Mini

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Lactic Acid Metabolic Compound Library

High-Throughput Bioactive Compound Library

Alle AMPK Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

MARK4 inhibitor 2 Related Classifications

HY-154986 Related Classifications