1070867-85-4
Chemical Structure
tris-NTA Biotin
- CAS No.: 1070867-85-4
- Formula:C59H93N11O25S
- Molecular Weight:1388.49
InChIKey: ROTBJHGOGUERSI-LNQQGTGFSA-N
SMILES: O=C(N1CCN(CCCN(CCN(CCC1)C(CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O)=O)C(CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O)=O)C(CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O)=O)CCCCCNC(CCCCCNC(CCCC[C@H]2[C@]3([H])[C@](NC(N3)=O)([H])CS2)=O)=O
Biological Activity: Tris-NTA Biotin is a biotin-labeled version of tris-NTA. Tris-NTA Biotin, as a bridge connecting His-tagged proteins with streptavidin, can reversibly label proteins and cell surfaces. Tris-NTA Biotin can be used in experiments such as protein immobilization, kinetic determination, live cell labeling, and Western blot detection[1][2].
| Cat. No. | Product Name | Purity | Description | Pricing | |||||||||||||||||||
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tris-NTA Biotin | 99.19% | Tris-NTA Biotin is a biotin-labeled version of tris-NTA. Tris-NTA Biotin, as a bridge connecting His-tagged proteins with streptavidin, can reversibly label proteins and cell surfaces. Tris-NTA Biotin can be used in experiments such as protein immobilization, kinetic determination, live cell labeling, and Western blot detection. | ||||||||||||||||||||
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References
- [1]. Huang Z, et al. Tris-nitrilotriacetic acids of subnanomolar affinity toward hexahistidine tagged molecules. Bioconjug Chem. 2009 Aug 19;20(8):1667-72. [Content Brief]
- [2]. Reichel A, et al. Noncovalent, site-specific biotinylation of histidine-tagged proteins. Anal Chem. 2007 Nov 15;79(22):8590-600. [Content Brief]
