tris-NTA Biotin
Based on 1 Customer Validation
Tris-NTA Biotin is a biotin-labeled version of tris-NTA. Tris-NTA Biotin, as a bridge connecting His-tagged proteins with streptavidin, can reversibly label proteins and cell surfaces. Tris-NTA Biotin can be used in experiments such as protein immobilization, kinetic determination, live cell labeling, and Western blot detection.
For research use only. We do not sell to patients.
- Purity: 99.19%
- CAS No.: 1070867-85-4
- Formula: C59H93N11O25S
- Molecular Weight:1388.49
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Biotin-tris-NTA is immobilized on streptavidin chips to form either low-density or high-density tris-NTA surfaces, enabling the stable and reversible immobilization of His6 proteins[1].
Biotin-tris-NTA can be used to develop a non-covalent, site-specific, reversible biotinylation method for the immobilization of His-tagged proteins at the interface, solution conjugation, live cell labeling, and Western blot detection[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1070867-85-4
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Appearance Solid
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Molecular Weight 1388.49
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Formula C59H93N11O25S
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Color White to off-white
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SMILES
O=C(N1CCN(CCCN(CCN(CCC1)C(CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O)=O)C(CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O)=O)C(CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O)=O)CCCCCNC(CCCCCNC(CCCC[C@H]2[C@]3([H])[C@](NC(N3)=O)([H])CS2)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : ≥ 100 mg/mL (72.02 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Huang Z, et al. Tris-nitrilotriacetic acids of subnanomolar affinity toward hexahistidine tagged molecules. Bioconjug Chem. 2009 Aug 19;20(8):1667-72. [Content Brief]
[2]. Reichel A, et al. Noncovalent, site-specific biotinylation of histidine-tagged proteins. Anal Chem. 2007 Nov 15;79(22):8590-600. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.7202 mL | 3.6010 mL | 7.2021 mL | 18.0052 mL |
| 5 mM | 0.1440 mL | 0.7202 mL | 1.4404 mL | 3.6010 mL | |
| 10 mM | 0.0720 mL | 0.3601 mL | 0.7202 mL | 1.8005 mL | |
| 15 mM | 0.0480 mL | 0.2401 mL | 0.4801 mL | 1.2003 mL | |
| 20 mM | 0.0360 mL | 0.1801 mL | 0.3601 mL | 0.9003 mL | |
| 25 mM | 0.0288 mL | 0.1440 mL | 0.2881 mL | 0.7202 mL | |
| 30 mM | 0.0240 mL | 0.1200 mL | 0.2401 mL | 0.6002 mL | |
| 40 mM | 0.0180 mL | 0.0900 mL | 0.1801 mL | 0.4501 mL | |
| 50 mM | 0.0144 mL | 0.0720 mL | 0.1440 mL | 0.3601 mL | |
| 60 mM | 0.0120 mL | 0.0600 mL | 0.1200 mL | 0.3001 mL |
tris-NTA Biotin Related Classifications
HY-D2355 Related Classifications
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