tris-NTA Biotin

Cat. No.: HY-D2355 Purity: 99.19%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Tris-NTA Biotin is a biotin-labeled version of tris-NTA. Tris-NTA Biotin, as a bridge connecting His-tagged proteins with streptavidin, can reversibly label proteins and cell surfaces. Tris-NTA Biotin can be used in experiments such as protein immobilization, kinetic determination, live cell labeling, and Western blot detection.

For research use only. We do not sell to patients.

tris-NTA Biotin Chemical Structure

CAS No. : 1070867-85-4

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Description

In Vitro

Biotin-tris-NTA is immobilized on streptavidin chips to form either low-density or high-density tris-NTA surfaces, enabling the stable and reversible immobilization of His6 proteins^[1].
Biotin-tris-NTA can be used to develop a non-covalent, site-specific, reversible biotinylation method for the immobilization of His-tagged proteins at the interface, solution conjugation, live cell labeling, and Western blot detection^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1388.49

Formula

C₅₉H₉₃N₁₁O₂₅S

CAS No.

1070867-85-4

Appearance

Solid

Color

White to off-white

SMILES

O=C(N1CCN(CCCN(CCN(CCC1)C(CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O)=O)C(CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O)=O)C(CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O)=O)CCCCCNC(CCCCCNC(CCCC[C@H]2[C@]3([H])[C@](NC(N3)=O)([H])CS2)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (72.02 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.7202 mL	3.6010 mL	7.2021 mL
5 mM	0.1440 mL	0.7202 mL	1.4404 mL
10 mM	0.0720 mL	0.3601 mL	0.7202 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.19%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (250 KB) HNMR (325 KB) RP-HPLC (247 KB) LCMS (237 KB)

Handling Instructions (2659 KB)

References

[1]. Huang Z, et al. Tris-nitrilotriacetic acids of subnanomolar affinity toward hexahistidine tagged molecules. Bioconjug Chem. 2009 Aug 19;20(8):1667-72. [Content Brief]

[2]. Reichel A, et al. Noncovalent, site-specific biotinylation of histidine-tagged proteins. Anal Chem. 2007 Nov 15;79(22):8590-600.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.7202 mL	3.6010 mL	7.2021 mL	18.0052 mL
	5 mM	0.1440 mL	0.7202 mL	1.4404 mL	3.6010 mL
	10 mM	0.0720 mL	0.3601 mL	0.7202 mL	1.8005 mL
	15 mM	0.0480 mL	0.2401 mL	0.4801 mL	1.2003 mL
	20 mM	0.0360 mL	0.1801 mL	0.3601 mL	0.9003 mL
	25 mM	0.0288 mL	0.1440 mL	0.2881 mL	0.7202 mL
	30 mM	0.0240 mL	0.1200 mL	0.2401 mL	0.6002 mL
	40 mM	0.0180 mL	0.0900 mL	0.1801 mL	0.4501 mL
	50 mM	0.0144 mL	0.0720 mL	0.1440 mL	0.3601 mL
	60 mM	0.0120 mL	0.0600 mL	0.1200 mL	0.3001 mL

tris-NTA Biotin Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

tris-NTA Biotin1070867-85-4Biochemical Assay ReagentsreversibleInhibitorinhibitorinhibit

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tris-NTA Biotin

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