rG4 mut_AHPC_PEG2
rG4 mut_AHPC_PEG2 is a structure-disruptive negative control PROTAC. rG4 mut_AHPC_PEG2 demonstrates that the RNA G-quadruplex conformation is an absolute prerequisite for the recruitment and degradation of DHX36 by retaining the E3-recruiting moiety (AHPC-PEG2) while disrupting the secondary structure of the nucleic acid moiety (mutated RNA G-quadruplex),.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
-
Stockage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
- Pureté et documentation
- Références
-
Help & FAQs
Voir tous les produits spécifiques à Isoform PROTACs
More
Activité biologique
rG4 mut_AHPC_PEG2 (30 nM; 30 min) does not bind to RHAU53 peptide, confirming that G4 structure integrity is required for interaction with DHX36 fragments[1].
rG4 mut_AHPC_PEG2 (3 pmol; 4 h) does not colocalize with endogenous DHX36 in HeLa cells, confirming it cannot target DHX36 in cells[1].
rG4 mut_AHPC_PEG2 (250 nM; 24 h) does not degrade DHX36 protein in HeLa cells[1].
rG4 mut_AHPC_PEG2 (0-200 nM; 48 h) does not inhibit translation of the APP rG4 WT reporter construct in HeLa cells[1].
rG4 mut_AHPC_PEG2 (0-100 nM; 24 h) does not reduce native APP protein expression or degrade DHX36 in HEK 293T cells[1].
rG4 mut_AHPC_PEG2 (200 nM; 24 h) does not degrade DHX36 or reduce Gnai2 protein expression in C2C12 myoblast cells[1].
rG4 mut_AHPC_PEG2 (200 nM; 24 h) does not impair the proliferative capacity of C2C12 myoblast cells or skeletal muscle stem cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:HeLa cells
-
Concentration:3 pmol (FAM-labeled rG4 mut_AHPC_PEG2)
-
Incubation Time:4 h (transfection)
-
Result:Observed little to no colocalized fluorescent foci between FAM-labeled rG4 mut_AHPC_PEG2 and endogenous DHX36.
-
Cell Line:HeLa cells
-
Concentration:250 nM
-
Incubation Time:24 h (transfection)
-
Result:Caused no significant reduction in DHX36 protein levels compared to untreated or empty transfection controls.
-
Cell Line:HEK 293T cells
-
Concentration:0-100 nM
-
Incubation Time:24 h (co-transfection)
-
Result:Did not reduce native APP or Myc protein levels.
Did not degrade DHX36 protein.
-
Cell Line:mouse C2C12 myoblast cells
-
Concentration:200 nM
-
Incubation Time:24 h (transfection)
-
Result:Did not degrade DHX36 protein.
Did not reduce Gnai2 protein levels compared to controls.
-
Cell Line:mouse C2C12 myoblast cells, skeletal muscle stem cells (SCs)
-
Concentration:200 nM (rG4 mut_AHPC_PEG2); 10 μM (EdU)
-
Incubation Time:24 h (rG4 mut_AHPC_PEG2 transfection); 3 h (EdU incubation)
-
Result:Showed 55.06% EdU-positive C2C12 cells with no inhibition of proliferation.
Showed 3.70% EdU-positive SCs with no inhibition of proliferation.
Chemical Information
-
Appearance Solid
-
SMILES
[rG4 mut_AHPC_PEG2]
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
rG4 mut_AHPC_PEG2 Related Classifications
HY-185724 Related Classifications
Powered by Bioz