Choloylglycine hydrolase
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Choloylglycine hydrolase (Bile salt hydrolase) is an important intestinal hydrolase. Choloylglycine hydrolase not only catalyzes the decomposition of conjugated bile acids into free bile acids and amino acids, but also exhibits amine N-acyltransferase activity, which re-links amino acids/amines to free bile acids (even conjugated bile acids) to produce bacterial bile acid amides. Choloylglycine hydrolase regulates the composition of the bile acid pool, intestinal mucosal barrier integrity, intestinal inflammatory responses, intestinal flora composition, host weight gain, circadian rhythm, and colonization resistance to Clostridioides difficile. Choloylglycine hydrolase can be used in the research of inflammatory bowel diseases, including ulcerative colitis and Crohn's disease.
For research use only. We do not sell to patients.
- CAS No.: 37289-07-9
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Choloylglycine hydrolase exhibits in vitro bile salt hydrolase activity that catalyzes the deconjugation of conjugated bile acids[1].
Choloylglycine hydrolase catalyzes the deconjugation of glycine- or taurine-conjugated primary bile acids via hydrolysis of the C-24 N-acyl bond, enabling downstream bacterial bile acid biotransformation[1].
Choloylglycine hydrolase (10 mg/mL; 90 min) acts as an amine N-acyltransferase to produce bacterial bile acid amidates from cholic acid and taurocholic acid in vitro[2].
Choloylglycine hydrolase (1 mM; 4 h) confers amine N-acyltransferase activity to Escherichia coli BL21(DE3), enabling production of bacterial bile acid amidates from cholic acid and taurocholic acid[2].
Choloylglycine hydrolase (1 mM; 16 h) is required for amine N-acyltransferase activity and bacterial bile acid amidate production in Bacteroides fragilis NCTC 9343[2].
Choloylglycine hydrolase (1 mM; 4 h) requires active site residues Cys1, Arg17, Asn172, and Arg225 for both the deconjugation and amine N-acyltransferase activities when expressed in Escherichia coli BL21(DE3)[2].
Choloylglycine hydrolase (200 μM) has its gene expression upregulated by 2.7-fold in Bifidobacterium longum NCTC 11818 upon treatment with total cholic acid and deoxycholic acid[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Bifidobacterium longum NCTC 11818
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Concentration:200 μM total CA and DCA
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Incubation Time:~6 h to mid-exponential phase
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Result:Increased bsh mRNA expression by 2.7-fold compared to vehicle control.
Confirmed RNA sequencing results showing significant upregulation.
Choloylglycine hydrolase (108 CFUs) expressed by wild-type B. fragilis drives bile acid hydrolysis and bacterial bile acid amidate production in the mouse gastrointestinal tract, significantly increasing unconjugated primary bile acids, free taurine, and bacterial bile acid amidates (BBAA) levels compared to mice colonized with a Δbsh strain[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
3.5.1.24
≥100 U/mg protein
One unit is defined as the amount of enzyme that will hydrolyze 1μmol of Glycocholic Acid to Glycine and Cholic Acid in five minutes at pH 5.6 at 37°C.
Chemical Information
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CAS No. 37289-07-9
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Appearance Solid
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Color White to off-white
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SMILES
[Choloylglycine hydrolase]
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Synonyms
Bile salt hydrolase
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Gadaleta RM, et al. Bile Salt Hydrolase-Competent Probiotics in the Management of IBD: Unlocking the "Bile Acid Code". Nutrients. 2022 Aug 05;14(15):3212. [Content Brief]
[2]. Rimal B, et al. Bile salt hydrolase catalyses formation of amine-conjugated bile acids. Nature. 2024 Feb;626(8000):859-863. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Choloylglycine hydrolase Related Classifications
HY-P2958 Related Classifications
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- Choloylglycine hydrolase
- 37289-07-9
- Bile salt hydrolase
- Acyltransferase
- conjugated bile acids
- inflammatory bowel disease
- ulcerative colitis
- Clostridium difficile
- Bifidobacterium longum NCTC 11818
- Bacteroides fragilis NCTC 9343
- Crohn’s disease
- unconjugated bile acids
- Escherichia coli BL21(DE3)
- Inhibitor
- inhibitor
- inhibit