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Alpha-Fetoprotein Protein, Human (HEK293, His)

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Based on 3 publication(s) in Google Scholar

Alpha-Fetoprotein, Human (HEK293, His) is a major plasma protein produced by the yolk sac and the fetal liver during fetal development.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Alpha-Fetoprotein Protein, Human (HEK293, His)
  • Alpha-Fetoprotein Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
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Biological Activity

Description

Alpha-Fetoprotein, Human (HEK293, His) is a major plasma protein produced by the yolk sac and the fetal liver during fetal development.

Background

Alpha-Fetoprotein is one of several tumor markers, such as hepatocellular carcinomas, cirrhosis, and chronic acute hepatitis. Tumor markers mean that molecules in the blood are higher when one has certain cancers[1].
Alpha-Fetoprotein can be detected in liver cancer, nonseminomatous germ cell tumors, cirrhosis or chronic active hepatitis lso have higher blood levels of AFP[1].

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P02771 (T20-V609)

  • Gene ID
    174  [NCBI]
  • Molecular Construction
    • N-term
    • Alpha-Fetoprotein (T20-V609)
      Accession # P02771
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    AFP; Alpha-Fetoglobulin; Prev. HPAFP; Fetoglobulin; Alpha-Fetoprotein; AFPD; Alpha-1-Fetoprotein; Alpha Fetoprotein; FETA

  • AA Sequence

    TLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSLLNQHACAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEHCCRGDVLDCLQDGEKIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAENDEKPEGLSPNLNRFLGDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLECQDKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV

  • Molecular Weight

    Approximately 65-70 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Jing-Yue Yang, et al. Co-transfection of Dendritic Cells With AFP and IL-2 Genes Enhances the Induction of Tumor Antigen-Specific Antitumor Immunity. Exp Ther Med. 2012 Oct;4(4):655-660. [Content Brief]

[2]. Mingyue Zhu, et al. Hepatitis B Virus X Protein Induces Expression of Alpha-Fetoprotein and Activates PI3K/mTOR Signaling Pathway in Liver Cells.Oncotarget. 2015 May 20;6(14):12196-208. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Alpha-Fetoprotein Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Alpha-Fetoprotein Protein, Human (HEK293, His)
  • Alpha-Fetoprotein Protein, Human (HEK293, His) Product Image