DSG3 Protein, Mouse (HEK293, His)

DSG3 protein facilitates cell-cell adhesion by mediating the interaction between plaque proteins and intermediate filaments at intercellular desmosome junctions. It interacts with PKP2, enhancing the stability and integrity of these junctions. DSG3 plays a vital role in maintaining tissue architecture and structural integrity through strong cell adhesion. DSG3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived DSG3 protein, expressed by HEK293 , with C-His labeled tag.

DSG3 protein is a vital component of intercellular desmosome junctions, playing a crucial role in facilitating cell-cell adhesion by mediating the interaction between plaque proteins and intermediate filaments. Notably, DSG3 interacts with PKP2, further contributing to the stability and integrity of these junctions. This protein is essential for maintaining the structural integrity of tissues by promoting strong cell adhesion and proper tissue architecture.

Measured by the ability of immobilized DSG3 to support the adhesion of Caki-2 human clear cell carcinoma epithelial cells. The ED₅₀ for this effect is 1.343 μg/mL, corresponding to a specific activity is 744.602 units/mg.

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  Measured by the ability of immobilized DSG3 to support the adhesion of Caki-2 human clear cell carcinoma epithelial cells. The ED₅₀ for this effect is 1.343 μg/mL, corresponding to a specific activity is 744.602 units/mg.

Mouse

HEK293

C-8*His

O35902-1 (E50-P617)

13512  [NCBI]

Extracellular Domain

EWVKFAKPCREREDNSRRNPIAKITSDFQKNQKITYRISGVGIDQPPFGIFVVDPNNGDINITAIVDREETPSFLITCRALNALGQDVERPLILTVKILDVNDNPPIFSQTIFKGEIEENSASNSLVMILNATDADEPNHMNSKIAFKIVSQEPAGMSMFLISRNTGEVRTLTSSLDREQISSYHLVVSGADNDGTGLSTQCECSIKIKDVNDNFPVLRESQYSARIEENTLNAELLRFQVTDWDEEYTDNWLAVYFFTSGNEGNWFEIETDPRTNEGILKVVKALDYEQVQSMQFSIAVRNKAEFHQSVISQYRVQSTPVTIQVIDVREGISFRPPSKTFTVQRGVSTNKLVGYILGTYQATDEDTGKAASSVRYVLGRNDGGLLVIDSKTAQIKFVKNIDRDSTFIVNKTISAEVLAIDENTGKTSTGTIYVEVPSFNENCPSVVLEKKDICTSSPSVTLSVRTLDRGKYTGPYTVSLEEQPLKLPVMWTITTLNATSALLQAQQQVSPGVYNVPVIVKDNQDGLCDTPESLTLTVCQCDDRSMCRAPIPSREPNTYGESSWRLGP

68-78 kDa

≥ 95%, as determined by Bis-Tris PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/hsc-3-cell.html