Diphenylamine hydrochloride
Based on 1 Customer Validation
Diphenylamine hydrochloride (N-Phenylaniline hydrochloride) is an antihyperglycemic agent with oral activity and a common structure in non-steroidal anti-inflammatory drugs (NSAIDs) that uncouples oxidative phosphorylation in mitochondria, leading to a decrease in hepatic cell ATP levels and causing liver cell damage. Diphenylamine hydrochloride is also an industrial antioxidant, a dyeing mordant, and is used in agriculture as an antifungal and antibacterial agent.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 537-67-7
- Formula: C12H12ClN
- Molecular Weight:205.69
-
Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Antioxidant Compound Library
HY-L037
-
Anti-diabetic Compound Library
HY-L040
-
Antifungal Compound Library
HY-L048
-
Antibacterial Compound Library
HY-L049
-
Orally Active Compound Library
HY-L061
-
FDA Approved & Pharmacopeial Drug Library
HY-L066
-
Plant-Sourced Natural Product Library
HY-L115
-
Radioprotector Library
HY-L178
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Natural Product Library Plus
HY-L021P
-
Bioactive Compound Library
HY-L001
-
Anti-Fibrosis Compound Library
HY-L185
-
RO5 Drug-like Natural Product Library
HY-L200
-
High-Throughput Bioactive Compound Library
HY-L205
-
High-Throughput Natural Product Library
HY-L206
-
Mass Spectrometry Natural Product Library
HY-L262
-
Natural Product and Natural Product-Like Compound Library
HY-L021M
-
Anti-Infection Compound Library
HY-L002
-
Natural Product Library
HY-L021
Biological Activity
Diphenylamine (10 mg/kg, orally, single dose) hydrochloride lowers blood sugar in hyperglycemic rabbits[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Hyperglycemic rabbit[2]
-
Dosage:10 mg/kg; single dose
-
Administration:Oral
-
Result:Reduced blood sugar by 61.4% two hours after administration.
Chemical Information
-
CAS No. 537-67-7
-
Appearance Solid
-
Molecular Weight 205.69
-
Formula C12H12ClN
-
Color White to off-white
-
SMILES
[H]Cl.C1(NC2=CC=CC=C2)=CC=CC=C1
-
Synonyms
N-Phenylaniline hydrochloride
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 250 mg/mL (1215.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (10.11 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (10.11 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (271 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. W E Alexander, et al. Metabolism of diphenylamine in the rat, rabbit and man. Food Cosmet Toxicol. 1965 Oct;3(4):571-9. [Content Brief]
[2]. M S Karawya, wt al. Diphenylamine, an antihyperglycemic agent from onion and tea. J Nat Prod. 1984 Sep-Oct;47(5):775-80. [Content Brief]
[3]. Safe S, et al. Identification of toxic impurities in commercial diphenylamine. Bull Environ Contam Toxicol. 1977 Feb;17(2):204-7. [Content Brief]
[4]. Masubuchi Y, et al. Possible mechanism of hepatocyte injury induced by diphenylamine and its structurally related nonsteroidal anti-inflammatory drugs. J Pharmacol Exp Ther. 2000 Mar;292(3):982-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.8617 mL | 24.3087 mL | 48.6173 mL | 121.5433 mL |
| 5 mM | 0.9723 mL | 4.8617 mL | 9.7235 mL | 24.3087 mL | |
| 10 mM | 0.4862 mL | 2.4309 mL | 4.8617 mL | 12.1543 mL | |
| 15 mM | 0.3241 mL | 1.6206 mL | 3.2412 mL | 8.1029 mL | |
| 20 mM | 0.2431 mL | 1.2154 mL | 2.4309 mL | 6.0772 mL | |
| 25 mM | 0.1945 mL | 0.9723 mL | 1.9447 mL | 4.8617 mL | |
| 30 mM | 0.1621 mL | 0.8103 mL | 1.6206 mL | 4.0514 mL | |
| 40 mM | 0.1215 mL | 0.6077 mL | 1.2154 mL | 3.0386 mL | |
| 50 mM | 0.0972 mL | 0.4862 mL | 0.9723 mL | 2.4309 mL | |
| 60 mM | 0.0810 mL | 0.4051 mL | 0.8103 mL | 2.0257 mL | |
| 80 mM | 0.0608 mL | 0.3039 mL | 0.6077 mL | 1.5193 mL | |
| 100 mM | 0.0486 mL | 0.2431 mL | 0.4862 mL | 1.2154 mL |
Diphenylamine hydrochloride Related Classifications
HY-N7133 Related Classifications
Powered by Bioz