FEAEHNLALQTL-GGGSC
FEAEHNLALQTL-GGGSC is a scrambled control peptide targeting the Glypican-3 (GPC3) polypeptide, with a GGGSC linker at its C-terminus. FEAEHNLALQTL-GGGSC serves as a negative control in both hepatoma cells and tumor-bearing mouse models, lacks specific targeting ability, and can be used for hepatocellular carcinoma-related research.
For research use only. We do not sell to patients.
- Formula: C74H115N21O26S
- Molecular Weight:1746.90
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Glypican‑3 |
FEAEHNLALQTL-GGGSC (50-200 μM; 30 min) does not compete with GPC3-specific peptide binding to Hep3B cells, confirming it acts as a non-targeting control[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Hep3B cells
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Concentration:0, 50, 100, 150 and 200 μM
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Incubation Time:30 min
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Result:The negative control peptide did not competitively inhibit the binding of the fluorescently labeled peptide to Hep3B cells at any concentration tested, confirming that the binding interaction was specifically mediated by the peptide sequence rather than non‑specific adsorption of the fluorescent dye.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Nude athymic mice (nu/nu, 4-6 weeks old, 20-25 grams, orthotopically implanted with ∼1×106 Hep3B cells)[1]
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Dosage:150 μM
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Administration:i.v.; single dose
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Result:The scrambled control peptide showed diffuse, low-intensity signal in the tumor region without forming focal accumulation that could delineate clear tumor margins, indicating an absence of GPC3-specific affinity.
At 1.5 hours post-injection, biodistribution analysis revealed that fluorescence intensity of the scrambled control peptide in tumor tissue was significantly lower than that of the specific GPC3-binding peptide, further confirming the lack of GPC3-mediated selective tumor uptake.
Chemical Information
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Molecular Weight 1746.90
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Formula C74H115N21O26S
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Sequence
Phe-Glu-Ala-Glu-His-Asn-Leu-Ala-Leu-Gln-Thr-Leu-Gly-Gly-Gly-Ser-Cys
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Sequence Shortening
FEAEHNLALQTLGGGSC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
FEAEHNLALQTL-GGGSC Related Classifications
HY-P11921 Related Classifications
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