YW2065
Based on 1 Customer Validation
YW2065 is an Axin-1 stabilizer. Axin-1 is a scaffolding protein that regulates proteasome degradation of β-catenin. YW2065 exhibits anti-colorectal cancer effects via dual activities of wnt/β-catenin signaling inhibition and AMPK activation.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.91%
- No. CAS: 2131223-85-1
- Fòrmula: C20H15BrN4O
- Peso molecular:407.26
-
Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Pureza y Documentación
- Referencias
-
Help & FAQs
-
Stem Cell Signaling Compound Library
HY-L017
-
Wnt/Hedgehog/Notch Compound Library
HY-L020
-
Anti-Cancer Compound Library
HY-L025
-
Anti-Aging Compound Library
HY-L034
-
Differentiation Inducing Compound Library
HY-L038
-
Cytoskeleton Compound Library
HY-L060
-
Neuroprotective Compound Library
HY-L070
-
Anti-Breast Cancer Compound Library
HY-L074
-
Transcription Factor-Targeted Library
HY-L090
-
Anti-Liver Cancer Compound Library
HY-L101
-
Anti-Colorectal Cancer Compound Library
HY-L103
-
Anti-Prostate Cancer Compound Library
HY-L124
-
Anti-Pulmonary Fibrosis Compound Library
HY-L125
-
Cancer Stem Cells Compound Library
HY-L135
-
Antihypertensive Compound Library
HY-L145
-
Extracellular Vesicles (EVs) Compound Library
HY-L168
-
Anti-Ovarian Cancer Compound Library
HY-L173
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Bioactive Compound Library
HY-L001
-
Anti-Gastric Cancer Compound Library
HY-L184
-
Anti-Fibrosis Compound Library
HY-L185
-
Cell Proliferation Compound Library
HY-L201
-
High-Throughput Bioactive Compound Library
HY-L205
Actividad biológica
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| K562 | IC50 |
8.9 μM
Compound: 2
|
Anti-leukemia activity in human K562 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay
Anti-leukemia activity in human K562 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay
|
[PMID: 35777269] |
| SW480 | GI50 |
2.4 μM
Compound: 1c; YW2065
|
Growth inhibition of human SW480 cells by Cell-titer-fluor reagent based assay
Growth inhibition of human SW480 cells by Cell-titer-fluor reagent based assay
|
[PMID: 31769984] |
| SW480 | IC50 |
1.15 nM
Compound: 1c; YW2065
|
Inhibition of Wnt/beta-Catenin signaling in human SW480 cells incubated for 24 hrs by TCF/LEF dual-luciferase reporter assay
Inhibition of Wnt/beta-Catenin signaling in human SW480 cells incubated for 24 hrs by TCF/LEF dual-luciferase reporter assay
|
[PMID: 31769984] |
| SW-620 | IC50 |
11.6 nM
Compound: 1c; YW2065
|
Growth inhibition of human SW620 cells incubated for 72 hrs by Cell-titer-fluor reagent based assay
Growth inhibition of human SW620 cells incubated for 72 hrs by Cell-titer-fluor reagent based assay
|
[PMID: 31769984] |
| SW-620 | IC50 |
67.8 nM
Compound: 1c; YW2065
|
Growth inhibition of human SW620 cells over-expressing constitutively active beta-catenin S33Y mutant incubated for 72 hrs by Cell-titer-fluor reagent based assay
Growth inhibition of human SW620 cells over-expressing constitutively active beta-catenin S33Y mutant incubated for 72 hrs by Cell-titer-fluor reagent based assay
|
[PMID: 31769984] |
| SW-620 | IC50 |
85.4 nM
Compound: 1c; YW2065
|
Growth inhibition of human SW620 cells transfected with lentivrus-encoding shRNA against Axin-1 incubated for 72 hrs by Cell-titer-fluor reagent based assay
Growth inhibition of human SW620 cells transfected with lentivrus-encoding shRNA against Axin-1 incubated for 72 hrs by Cell-titer-fluor reagent based assay
|
[PMID: 31769984] |
Chemical Information
-
No. CAS 2131223-85-1
-
Appearance Solid
-
Peso molecular 407.26
-
Fòrmula C20H15BrN4O
-
Color Off-white to light yellow
-
SMILES
O=C(C1=C(C)N(C2=CC=CC=C2Br)N=C1)NC3=NC4=CC=CC=C4C=C3
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 100 mg/mL (245.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.14 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
-
Ficha de datos (272 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4554 mL | 12.2772 mL | 24.5543 mL | 61.3858 mL |
| 5 mM | 0.4911 mL | 2.4554 mL | 4.9109 mL | 12.2772 mL | |
| 10 mM | 0.2455 mL | 1.2277 mL | 2.4554 mL | 6.1386 mL | |
| 15 mM | 0.1637 mL | 0.8185 mL | 1.6370 mL | 4.0924 mL | |
| 20 mM | 0.1228 mL | 0.6139 mL | 1.2277 mL | 3.0693 mL | |
| 25 mM | 0.0982 mL | 0.4911 mL | 0.9822 mL | 2.4554 mL | |
| 30 mM | 0.0818 mL | 0.4092 mL | 0.8185 mL | 2.0462 mL | |
| 40 mM | 0.0614 mL | 0.3069 mL | 0.6139 mL | 1.5346 mL | |
| 50 mM | 0.0491 mL | 0.2455 mL | 0.4911 mL | 1.2277 mL | |
| 60 mM | 0.0409 mL | 0.2046 mL | 0.4092 mL | 1.0231 mL | |
| 80 mM | 0.0307 mL | 0.1535 mL | 0.3069 mL | 0.7673 mL | |
| 100 mM | 0.0246 mL | 0.1228 mL | 0.2455 mL | 0.6139 mL |
YW2065 Related Classifications
HY-141543 Related Classifications
Powered by Bioz