1. Stem Cell/Wnt
  2. Wnt
    β-catenin
  3. SKL2001

SKL2001 

Cat. No.: HY-101085 Purity: 99.54%
Handling Instructions

SKL2001 is an agonist of the Wnt/β-catenin pathway, with anti-cancer activity. SKL2001 stabilizes intracellular β-catenin via disruption of the Axin/β-catenin interaction.

For research use only. We do not sell to patients.

SKL2001 Chemical Structure

SKL2001 Chemical Structure

CAS No. : 909089-13-0

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Free Sample (0.5-1 mg)   Apply Now  
10 mM * 1 mL in DMSO USD 77 In-stock
Estimated Time of Arrival: December 31
10 mg USD 70 In-stock
Estimated Time of Arrival: December 31
50 mg USD 290 In-stock
Estimated Time of Arrival: December 31
100 mg USD 520 In-stock
Estimated Time of Arrival: December 31
200 mg USD 820 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 12 publication(s) in Google Scholar

Top Publications Citing Use of Products

    SKL2001 purchased from MCE. Usage Cited in: Mol Ther Nucleic Acids. 2020 Jun 27;21:577-591.

    Western blotting confirmed that β-catenin signaling is activated after GC cells are cultured with SKL2001 (40 μM), accompanied by the enhanced expression of the downstream molecules Cyclin D1, c-Myc, and nuclear β-catenin in BGC-823 cells.
    • Biological Activity

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    Description

    SKL2001 is an agonist of the Wnt/β-catenin pathway, with anti-cancer activity. SKL2001 stabilizes intracellular β-catenin via disruption of the Axin/β-catenin interaction[1].

    IC50 & Target

    Wnt/β-catenin[1]

    In Vitro

    SKL2001 is an agonist of the Wnt/β-catenin pathway, and also upregulates the expression of Axin2, a downstream target of the Wnt/β-catenin pathway, but shows no effect on NF-κB, p53 reporter activity and GSK-3β activity. SKL2001 causes osteoblast differentiation (20 and 40 μM) and suppresses preadipocyte differentiation (5, 10, and 30 μM) via the activation of the Wnt/β-catenin pathway. SKL2001 (5, 10, and 30 μM) stabilizes intracellular β-catenin in 3T3-L1 cells[1]. SKL2001 (40 μM) significantly inhibits the proliferation of HCT116 spheroids independently of cytotoxicity and the inhibition is reversible; SKL2001 cuases cell cycle arrest in HCT116 spheroids. SKL2001 (40 μM) enhances round-shape spheroid formation and E-cadherin expression[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    286.29

    Formula

    C₁₄H₁₄N₄O₃

    CAS No.

    909089-13-0

    SMILES

    O=C(C1=NOC(C2=CC=CO2)=C1)NCCCN3C=CN=C3

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 96.66 mg/mL (337.63 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.4930 mL 17.4648 mL 34.9296 mL
    5 mM 0.6986 mL 3.4930 mL 6.9859 mL
    10 mM 0.3493 mL 1.7465 mL 3.4930 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (8.73 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (8.73 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (8.73 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Cell Assay
    [1]

    ST2 cells are cultured on glass chamber slides and then treated with DMSO or SKL2001 for 15 h. After treatment, the cells are washed with PBS, fixed with 4% formaldehyde, permeabilized in 0.3% Triton X-100, and blocked in 4% bovine serum albumin for 1 h. The cells are stained with anti-β-catenin antibody and then analyzed by confocal microscopy using a microscope[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Keywords:

    SKL2001SKL 2001SKL-2001Wntβ-cateninBeta cateninInhibitorinhibitorinhibit

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    Product Name:
    SKL2001
    Cat. No.:
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