H2S scavenger 1 triflate
Based on 1 Customer Validation
H2S scavenger 1 triflate is a selective H2S scavenger and antibacterial adjuvant. H2S scavenger 1 triflate consumes hydrogen sulfide produced by H2S-producing bacteria via chemical scavenging, and does not act on H2S synthases. H2S scavenger 1 triflate enhances the clearance of H2S-producing bacteria mediated by macrophages and polymorphonuclear neutrophils. H2S scavenger 1 triflate inhibits the biofilm formation of H2S-producing bacteria and eliminates pre-formed biofilms. H2S scavenger 1 triflate can be used for the research of Pseudomonas aeruginosa-infected pneumonia and Pseudomonas aeruginosa-infected skin wounds.
For research use only. We do not sell to patients.
- Purity: 99.69%
- Formula: C13H16F3N5O6S
- Molecular Weight:427.36
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
H2S scavenger 1 triflate (Compound 7b) (110 µM; intervals at room temperature) potently scavenges H2S in a cell-free buffer, reducing H2S decay half-life to 3 minutes[1].
H2S scavenger 1 triflate (3.125-100 μM; 12 h at 37 °C) dose-dependently scavenges H2S produced by S. aureus[1].
H2S scavenger 1 triflate (0-200 μM; 24 h at 37 °C, 5% CO2) is non-toxic to HEK 293 cells at concentrations up to 200 μM and does not enhance antibiotic cytotoxicity in these cells[1].
H2S scavenger 1 triflate (25 μM; up to 2 h at 37 °C) significantly sensitizes S. aureus, MRSA, E. coli, and wild-type P. aeruginosa to multiple antibiotics by depleting bacterial H2S, with no effect on H2S-deficient cbs/cse knockout P. aeruginosa[1].
H2S scavenger 1 triflate (25 μM; 5 min at room temperature, followed by photoirradiation) significantly sensitizes S. aureus, P. aeruginosa, E. coli, and MRSA to photodynamic therapy with Ce6 and 665 nm light[1].
H2S scavenger 1 triflate (6.25-25 μM; 0.5, 1, 2 h at 37 °C, 5% CO2) dose-dependently enhances RAW 264.7 macrophage clearance of S. aureus, E. coli, and wild-type P. aeruginosa by depleting bacterial H2S, with no effect on H2S-deficient cbs/cse knockout P. aeruginosa at 1 and 2 hours[1].
H2S scavenger 1 triflate (6.25-25 μM; 24 h at 37 °C) dose-dependently inhibits biofilm formation in S. aureus and wild-type P. aeruginosa by depleting bacterial H2S, with no effect on H2S-deficient cbs/cse knockout P. aeruginosa[1].
H2S scavenger 1 triflate (6.25-25 μM; 24 h at 37 °C on pre-formed biofilms) dose-dependently eradicates pre-formed biofilms in S. aureus and wild-type P. aeruginosa by depleting bacterial H2S, with no effect on H2S-deficient cbs/cse knockout P. aeruginosa[1].
H2S scavenger 1 triflate (25 μM; up to 28 h at 37 °C) significantly sensitizes S. aureus and wild-type P. aeruginosa persister cells to antibiotics by depleting bacterial H2S, with no effect on H2S-deficient cbs/cse knockout P. aeruginosa persisters[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEK 293 cells
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Concentration:0-200 μM
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Incubation Time:24 h at 37 °C, 5% CO2
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Result:Showed no obvious cytotoxicity toward HEK 293 cells at concentrations up to 200 μM, and did not increase the cytotoxicity of tested antibiotics.
H2S scavenger 1 triflate (20 mg/kg; s.c.; single dose) does not potentiate gentamicin's antibacterial activity in cbs/cse knockout Pseudomonas aeruginosa-infected pneumonia mice, confirming its activity is dependent on bacterial H2S depletion[1].
H2S scavenger 1 triflate (20 mg/kg; local wound injection; 3 doses (at 24, 48, 72 hours post-infection)) potentiates gentamicin's antibacterial activity and improves wound healing in wild-type Pseudomonas aeruginosa-infected skin wound mice, while monotherapy with the reagent also reduces bacterial burden and promotes wound healing[1].
H2S scavenger 1 triflate (20 mg/kg; local wound injection; 3 doses (at 24, 48, 72 hours post-infection)) does not potentiate gentamicin's antibacterial activity or improve wound healing in cbs/cse knockout Pseudomonas aeruginosa-infected skin wound mice, confirming its activity is dependent on bacterial H2S depletion[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c (female, 6-8 weeks old, 18-22 g, intranasal inoculation with wild-type Pseudomonas aeruginosa)[1]
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Dosage:20 mg/kg (combination with gentamicin); 20 mg/kg (monotherapy)
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Administration:s.c.; single dose
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Result:Significantly reduced bacterial burden in both lung homogenate and bronchoalveolar lavage fluid (BALF) when coadministered with gentamicin compared to gentamicin monotherapy.
Did not reduce bacterial colonies when used as monotherapy compared to the vehicle group.
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Animal Model:BALB/c (female, 6-8 weeks old, 18-22 g, dorsal wounds injected with wild-type Pseudomonas aeruginosa)[1]
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Dosage:20 mg/kg (combination with gentamicin); 20 mg/kg (monotherapy)
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Administration:local wound injection; 3 doses (at 24, 48, 72 hours post-infection)
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Result:Reduced bacterial burden and wound size significantly when coadministered with gentamicin compared to gentamicin monotherapy.
Reduced bacterial colonies and wound size when used as monotherapy compared to the vehicle group.
Chemical Information
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Appearance Solid
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Molecular Weight 427.36
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Formula C13H16F3N5O6S
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Color Light yellow to yellow
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SMILES
C[N+](CC1)(C)CCN1C2=CC=C([N+]([O-])=O)C3=NON=C23.O=S(C(F)(F)F)([O-])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (233.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (11.70 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (11.70 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (284 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3399 mL | 11.6997 mL | 23.3995 mL | 58.4987 mL |
| 5 mM | 0.4680 mL | 2.3399 mL | 4.6799 mL | 11.6997 mL | |
| 10 mM | 0.2340 mL | 1.1700 mL | 2.3399 mL | 5.8499 mL | |
| 15 mM | 0.1560 mL | 0.7800 mL | 1.5600 mL | 3.8999 mL | |
| 20 mM | 0.1170 mL | 0.5850 mL | 1.1700 mL | 2.9249 mL | |
| 25 mM | 0.0936 mL | 0.4680 mL | 0.9360 mL | 2.3399 mL | |
| 30 mM | 0.0780 mL | 0.3900 mL | 0.7800 mL | 1.9500 mL | |
| 40 mM | 0.0585 mL | 0.2925 mL | 0.5850 mL | 1.4625 mL | |
| 50 mM | 0.0468 mL | 0.2340 mL | 0.4680 mL | 1.1700 mL | |
| 60 mM | 0.0390 mL | 0.1950 mL | 0.3900 mL | 0.9750 mL | |
| 80 mM | 0.0292 mL | 0.1462 mL | 0.2925 mL | 0.7312 mL | |
| 100 mM | 0.0234 mL | 0.1170 mL | 0.2340 mL | 0.5850 mL |
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