H2S scavenger 1 triflate

Name: H2S scavenger 1 triflate
Brand: MedChemExpress
SKU: HY-169331
Rating: 4.5 (1 reviews)

Cat. No.: HY-169331 Purity: 99.69%: Data Sheet
COA

SDS
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H2S scavenger 1 triflate is a selective H₂S scavenger and antibacterial adjuvant. H2S scavenger 1 triflate consumes hydrogen sulfide produced by H₂S-producing bacteria via chemical scavenging, and does not act on H₂S synthases. H2S scavenger 1 triflate enhances the clearance of H₂S-producing bacteria mediated by macrophages and polymorphonuclear neutrophils. H2S scavenger 1 triflate inhibits the biofilm formation of H₂S-producing bacteria and eliminates pre-formed biofilms. H2S scavenger 1 triflate can be used for the research of Pseudomonas aeruginosa-infected pneumonia and Pseudomonas aeruginosa-infected skin wounds.

For research use only. We do not sell to patients.

H2S scavenger 1 triflate Chemical Structure

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

H2S scavenger 1 triflate (Compound 7b) (110 µM; intervals at room temperature) potently scavenges H₂S in a cell-free buffer, reducing H₂S decay half-life to 3 minutes^[1].
H2S scavenger 1 triflate (3.125-100 μM; 12 h at 37 °C) dose-dependently scavenges H₂S produced by S. aureus^[1].
H2S scavenger 1 triflate (0-200 μM; 24 h at 37 °C, 5% CO₂) is non-toxic to HEK 293 cells at concentrations up to 200 μM and does not enhance antibiotic cytotoxicity in these cells^[1].
H2S scavenger 1 triflate (25 μM; up to 2 h at 37 °C) significantly sensitizes S. aureus, MRSA, E. coli, and wild-type P. aeruginosa to multiple antibiotics by depleting bacterial H₂S, with no effect on H₂S-deficient cbs/cse knockout P. aeruginosa^[1].
H2S scavenger 1 triflate (25 μM; 5 min at room temperature, followed by photoirradiation) significantly sensitizes S. aureus, P. aeruginosa, E. coli, and MRSA to photodynamic therapy with Ce6 and 665 nm light^[1].
H2S scavenger 1 triflate (6.25-25 μM; 0.5, 1, 2 h at 37 °C, 5% CO₂) dose-dependently enhances RAW 264.7 macrophage clearance of S. aureus, E. coli, and wild-type P. aeruginosa by depleting bacterial H₂S, with no effect on H₂S-deficient cbs/cse knockout P. aeruginosa at 1 and 2 hours^[1].
H2S scavenger 1 triflate (6.25-25 μM; 24 h at 37 °C) dose-dependently inhibits biofilm formation in S. aureus and wild-type P. aeruginosa by depleting bacterial H₂S, with no effect on H₂S-deficient cbs/cse knockout P. aeruginosa^[1].
H2S scavenger 1 triflate (6.25-25 μM; 24 h at 37 °C on pre-formed biofilms) dose-dependently eradicates pre-formed biofilms in S. aureus and wild-type P. aeruginosa by depleting bacterial H₂S, with no effect on H₂S-deficient cbs/cse knockout P. aeruginosa^[1].
H2S scavenger 1 triflate (25 μM; up to 28 h at 37 °C) significantly sensitizes S. aureus and wild-type P. aeruginosa persister cells to antibiotics by depleting bacterial H₂S, with no effect on H₂S-deficient cbs/cse knockout P. aeruginosa persisters^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:	HEK 293 cells
Concentration:	0-200 μM
Incubation Time:	24 h at 37 °C, 5% CO₂
Result:	Showed no obvious cytotoxicity toward HEK 293 cells at concentrations up to 200 μM, and did not increase the cytotoxicity of tested antibiotics.

In Vivo

H2S scavenger 1 triflate (20 mg/kg; s.c.; single dose) when coadministered with gentamicin significantly potentiates gentamicin's antibacterial activity in wild-type Pseudomonas aeruginosa-infected pneumonia mice, while monotherapy with 20 mg/kg of the reagent shows no bacterial clearance effect^[1].
H2S scavenger 1 triflate (20 mg/kg; s.c.; single dose) does not potentiate gentamicin's antibacterial activity in cbs/cse knockout Pseudomonas aeruginosa-infected pneumonia mice, confirming its activity is dependent on bacterial H₂S depletion^[1].
H2S scavenger 1 triflate (20 mg/kg; local wound injection; 3 doses (at 24, 48, 72 hours post-infection)) potentiates gentamicin's antibacterial activity and improves wound healing in wild-type Pseudomonas aeruginosa-infected skin wound mice, while monotherapy with the reagent also reduces bacterial burden and promotes wound healing^[1].
H2S scavenger 1 triflate (20 mg/kg; local wound injection; 3 doses (at 24, 48, 72 hours post-infection)) does not potentiate gentamicin's antibacterial activity or improve wound healing in cbs/cse knockout Pseudomonas aeruginosa-infected skin wound mice, confirming its activity is dependent on bacterial H₂S depletion^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c (female, 6-8 weeks old, 18-22 g, intranasal inoculation with wild-type Pseudomonas aeruginosa)^[1]
Dosage:	20 mg/kg (combination with gentamicin); 20 mg/kg (monotherapy)
Administration:	s.c.; single dose
Result:	Significantly reduced bacterial burden in both lung homogenate and bronchoalveolar lavage fluid (BALF) when coadministered with gentamicin compared to gentamicin monotherapy. Did not reduce bacterial colonies when used as monotherapy compared to the vehicle group.

Animal Model:	BALB/c (female, 6-8 weeks old, 18-22 g, dorsal wounds injected with wild-type Pseudomonas aeruginosa)^[1]
Dosage:	20 mg/kg (combination with gentamicin); 20 mg/kg (monotherapy)
Administration:	local wound injection; 3 doses (at 24, 48, 72 hours post-infection)
Result:	Reduced bacterial burden and wound size significantly when coadministered with gentamicin compared to gentamicin monotherapy. Reduced bacterial colonies and wound size when used as monotherapy compared to the vehicle group.

Molecular Weight

427.36

Formula

C₁₃H₁₆F₃N₅O₆S

Appearance

Solid

Color

Light yellow to yellow

SMILES

C[N+](CC1)(C)CCN1C2=CC=C([N+]([O-])=O)C3=NON=C23.O=S(C(F)(F)F)([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (233.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3399 mL	11.6997 mL	23.3995 mL
5 mM	0.4680 mL	2.3399 mL	4.6799 mL
10 mM	0.2340 mL	1.1700 mL	2.3399 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (11.70 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (11.70 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (11.70 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.69%

Select Batch:

Data Sheet (284 KB) SDS (251 KB)

COA (256 KB) HNMR (185 KB) RP-HPLC (178 KB) LCMS (239 KB)

Handling Instructions (2659 KB)

References

[1]. Sun J, et al. H₂S scavenger as a broad-spectrum strategy to deplete bacteria-derived H₂S for antibacterial sensitization. Nat Commun. 2024;15(1):9422. Published 2024 Oct 31. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3399 mL	11.6997 mL	23.3995 mL	58.4987 mL
	5 mM	0.4680 mL	2.3399 mL	4.6799 mL	11.6997 mL
	10 mM	0.2340 mL	1.1700 mL	2.3399 mL	5.8499 mL
	15 mM	0.1560 mL	0.7800 mL	1.5600 mL	3.8999 mL
	20 mM	0.1170 mL	0.5850 mL	1.1700 mL	2.9249 mL
	25 mM	0.0936 mL	0.4680 mL	0.9360 mL	2.3399 mL
	30 mM	0.0780 mL	0.3900 mL	0.7800 mL	1.9500 mL
	40 mM	0.0585 mL	0.2925 mL	0.5850 mL	1.4625 mL
	50 mM	0.0468 mL	0.2340 mL	0.4680 mL	1.1700 mL
	60 mM	0.0390 mL	0.1950 mL	0.3900 mL	0.9750 mL
	80 mM	0.0292 mL	0.1462 mL	0.2925 mL	0.7312 mL
	100 mM	0.0234 mL	0.1170 mL	0.2340 mL	0.5850 mL