Isorhamnetin-3-O-neohespeidoside
Based on 4 publication(s) in Google Scholar
Isorhamnetin-3-O-neohespeidoside is a flavonoid. Isorhamnetin-3-O-neohespeidoside can be isolated from Typha angustifolia. Isorhamnetin-3-O-neohespeidoside inhibits xanthine oxidase activity with an IC50 of 48.75 μg mL. Isorhamnetin-3-O-neohespeidoside has antioxidant and osteoclastogenic activities. Isorhamnetin-3-O-neohespeidoside can be used in research of bone.
For research use only. We do not sell to patients.
- Purity : 99.93%
- CAS No.: 55033-90-4
- Formula: C28H32O16
- Molecular Weight:624.54
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Isorhamnetin-3-O-neohespeidoside
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Biological Activity
Description
IC50 & Target
xanthine oxidase: IC50 of 48.75 μg mL
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Bel-7402 | IC50 |
74.4 μM
Compound: 2
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Cytotoxicity against human Bel7402 cells
Cytotoxicity against human Bel7402 cells
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10.1007/s00044-013-0773-3 |
| Caco-2 | IC50 |
2.47 μg/mL
Compound: 2
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Cytotoxicity against human Caco2 cells after 48 hrs by MTT assay
Cytotoxicity against human Caco2 cells after 48 hrs by MTT assay
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10.1007/s00044-013-0773-3 |
| HCT-116 | IC50 |
72 μM
Compound: 2
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Cytotoxicity against human HCT116 cells
Cytotoxicity against human HCT116 cells
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10.1007/s00044-013-0773-3 |
| NIH3T3 | IC50 |
>200 μg/mL
Compound: 2
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Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by MTT assay
Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by MTT assay
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10.1007/s00044-013-0773-3 |
| Raji | IC50 |
574 molar ratio
Compound: 11
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Inhibition of TPA-induced EBV-early antigen activation in human Raji cells relative to TPA
Inhibition of TPA-induced EBV-early antigen activation in human Raji cells relative to TPA
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[PMID: 17190444] |
| T47D | IC50 |
0.003 μg/mL
Compound: 2
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Cytotoxicity against human T47D cells after 48 hrs by MTT assay
Cytotoxicity against human T47D cells after 48 hrs by MTT assay
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10.1007/s00044-013-0773-3 |
In Vitro
Isorhamnetin-3-O-neohespeidoside (greater than 0.025 mg/mL) scavenges ABTS+• free radicals in a concentration-dependent manner with an IC50 of 0.06 mg/mL[2].
Isorhamnetin-3-O-neohespeidoside (19-150 μg/mL) inhibits xanthine oxidase activity with an IC50 of 48.75 μg mL and scavenges superoxide anions with an IC50 of 30 μg mL[2].
Isorhamnetin-3-O-neohespeidoside (1-50 μM; 4 days) promotes RANKL-induced osteoclastogenesis in BMMs in a dose-dependent manner, increasing the number and area of TRAP-positive osteoclasts[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 55033-90-4
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Appearance Solid
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Molecular Weight 624.54
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Formula C28H32O16
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Color Light yellow to yellow
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SMILES
O=C1C(O[C@H](O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H]2O[C@@](O[C@@H](C)[C@H](O)[C@H]3O)([H])[C@@H]3O)=C(C4=CC(OC)=C(O)C=C4)OC5=CC(O)=CC(O)=C15
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (4)
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Journal Impact Factor
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Most Recent
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Food Chem
Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]2025 Dec 30:497:146992. PMID: 41285060 -
Food Chem
Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]2025 Oct 15:489:144992. PMID: 40466530 -
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Solvent & Solubility
In Vitro:
DMSO : 250 mg/mL (400.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.33 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.33 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Wang X, et al. A rapid and efficient extraction method based on industrial MCM-41-miniaturized matrix solid-phase dispersion extraction with response surface methodology for simultaneous quantification of six flavonoids in Pollen typhae by ultra-high-performance liquid chromatography. J Sep Sci. 2019 Jul;42(14):2426-2434. [Content Brief]
[2]. Bouhlel I, et al. Assessment of isorhamnetin 3-O-neohesperidoside from Acacia salicina: protective effects toward oxidation damage and genotoxicity induced by aflatoxin B1 and nifuroxazide. J Appl Toxicol. 2010 Aug;30(6):551-8. [Content Brief]
[3]. Yu X, et al. Isorhamnetin 3-O-neohesperidoside promotes the resorption of crown-covered bone during tooth eruption by osteoclastogenesis. Sci Rep. 2020 Mar 20;10(1):5172. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6012 mL | 8.0059 mL | 16.0118 mL | 40.0295 mL |
| 5 mM | 0.3202 mL | 1.6012 mL | 3.2024 mL | 8.0059 mL | |
| 10 mM | 0.1601 mL | 0.8006 mL | 1.6012 mL | 4.0029 mL | |
| 15 mM | 0.1067 mL | 0.5337 mL | 1.0675 mL | 2.6686 mL | |
| 20 mM | 0.0801 mL | 0.4003 mL | 0.8006 mL | 2.0015 mL | |
| 25 mM | 0.0640 mL | 0.3202 mL | 0.6405 mL | 1.6012 mL | |
| 30 mM | 0.0534 mL | 0.2669 mL | 0.5337 mL | 1.3343 mL | |
| 40 mM | 0.0400 mL | 0.2001 mL | 0.4003 mL | 1.0007 mL | |
| 50 mM | 0.0320 mL | 0.1601 mL | 0.3202 mL | 0.8006 mL | |
| 60 mM | 0.0267 mL | 0.1334 mL | 0.2669 mL | 0.6672 mL | |
| 80 mM | 0.0200 mL | 0.1001 mL | 0.2001 mL | 0.5004 mL | |
| 100 mM | 0.0160 mL | 0.0801 mL | 0.1601 mL | 0.4003 mL |
Isorhamnetin-3-O-neohespeidoside Related Classifications
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