Cat. No.: HY-N1941

Isosinensetin

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Isosinensetin is a flavonoid compound and an inhibitor of HIV-1 protease and PTP1B (IC₅₀: 2.61 µM; K_i: 0.92 µM). Isosinensetin inhibits P-glycoprotein (P-gp) in MDR1-MDCKII cells. Isosinensetin has multiple activities such as anti-tumor, anti-viral, anti-inflammatory, and antioxidant effects. Isosinensetin can be used in the research of various diseases including cancer, inflammation, osteoporosis, diabetes, etc.

For research use only. We do not sell to patients.

Purity: 99.90%
CAS No.: 17290-70-9
Formula: C₂₀H₂₀O₇
Molecular Weight:372.37

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Isosinensetin Chemical Structure

CAS No.: 17290-70-9

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Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All NF-κB Isoforms :

All p38 MAPK Isoforms :

All ERK Isoforms :

All JNK Isoforms :

All Calcium Channel Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
3T3-L1	IC₅₀	43 μM Compound: 6	Anticorpulence activity against mouse 3T3L1 cells assessed as inhibition of lipid droplet accumulation Anticorpulence activity against mouse 3T3L1 cells assessed as inhibition of lipid droplet accumulation	[PMID: 19054677]
HT-29	IC₅₀	28 μM Compound: 6	Anticancer activity against human HT-29 cells after 72 hrs by MTT assay Anticancer activity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 19054677]
Monocyte	IC₅₀	16 μM Compound: 5,7,8,3',4'-pentamethoxyflavone	Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA	[PMID: 10096854]

In Vitro

Isosinensetin (0.1-100 μM; 4-24 h) can significantly inhibit P-gp (IC₅₀: 4.2 μM), and increase the cytotoxicity induced by paraquat in MDR1-MDCKII cells^[1].
Isosinensetin (250 μg/mL) can inhibit erythrocyte hemolysis with an inhibition rate of 92.08%^[2].
Isosinensetin (40-80 μM; 1 h) can increase the intracellular calcium ion concentration, promote the expression of hTAS2R50 and proglucagon, and stimulate the secretion of GLP-1 in NCI-H716 cells ^[3].
Isosinensetin (0-10 μM; 0-7 days) can inhibit the formation of osteoclasts and their bone resorption function in BMMs. The mechanism involves the inhibition of the ROS-mediated NF-κB/MAPK signaling pathway^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[4]

Cell Line:

Bone marrowderived macrophages treated RANKL
Concentration:

10 μM
Incubation Time:

0, 5, 10, 20, 30 and 60 min
Result:

Lowered the phosphorylation of ERK and P38 at the 10-minute and 20-minute time points, respectively.
Blocked JNK phosphorylation at both the 10-minute and 20-minute time points.

In Vivo

Isosinensetin (5-10 mg/kg; intraperitoneal injection; once every two days; 6 weeks) alleviates estrogen deficiency-induced osteoporosis in mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Ovariectomized (OVX) female C57BL/6 J mice^[4]
Dosage:

5 and 10 mg/kg
Administration:

Intraperitoneal injection; once every two days for 6 weeks
Result:

Significantly alleviated bone loss in OVX mice.
Increased bone volume per tissue volume (BV/TV), trabecular number (Tb.N), and trabecular thickness (Tb.Th), and decreased trabecular separation (Tb.Sp).
Reduced the number and area of TRAP-positive cells, indicating the inhibition of osteoclast activity.
Activated Nrf2-related antioxidant enzymes, such as HO-1 and CAT, and inhibited the expression of osteoclast-associated protein CTSK.

Chemical Information

CAS No. 17290-70-9
Appearance Solid
Molecular Weight 372.37
Formula C₂₀H₂₀O₇
Color Off-white to light yellow
SMILES

O=C1C=C(C2=CC=C(OC)C(OC)=C2)OC3=C(OC)C(OC)=CC(OC)=C13
Structure Classification
- Flavonoids
- Flavones
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 12.5 mg/mL (33.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6855 mL

13.4275 mL

26.8550 mL

5 mM

0.5371 mL

2.6855 mL

5.3710 mL

10 mM

0.2686 mL

1.3428 mL

2.6855 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bai J, et al. Inhibitory effects of flavonoids on P-glycoprotein in vitro and in vivo: Food/herb-drug interactions and structure-activity relationships. Toxicol Appl Pharmacol. 2019 Apr 15;369:49-59. [Content Brief]

[2]. Jiang Y, et al. Isolation of adenosine, iso-sinensetin and dimethylguanosine with antioxidant and HIV-1 protease inhibiting activities from fruiting bodies of Cordyceps militaris. Phytomedicine. 2011 Jan 15;18(2-3):189-93. [Content Brief]

[3]. Lee SH, et al. Isosinensetin Stimulates Glucagon-like Peptide-1 Secretion via Activation of hTAS2R50 and the Gβγ-Mediated Signaling Pathway. Int J Mol Sci. 2023 Feb 12;24(4):3682. [Content Brief]

[4]. Qin Y, et al. Isosinensetin alleviates estrogen deficiency-induced osteoporosis via suppressing ROS-mediated NF-κB/MAPK signaling pathways. Biomed Pharmacother. 2023 Apr;160:114347. [Content Brief]

[5]. Trang N M, et al. Inhibition of PTP1B by isosinensetin, a polymethoxylated flavone isolated from trifoliate orange peel: Kinetic studies, molecular docking, and molecular dynamics simulation. Chemical Papers, 2023, 77(3): 1751-1757.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6855 mL	13.4275 mL	26.8550 mL	67.1375 mL
	5 mM	0.5371 mL	2.6855 mL	5.3710 mL	13.4275 mL
	10 mM	0.2686 mL	1.3428 mL	2.6855 mL	6.7138 mL
	15 mM	0.1790 mL	0.8952 mL	1.7903 mL	4.4758 mL
	20 mM	0.1343 mL	0.6714 mL	1.3428 mL	3.3569 mL
	25 mM	0.1074 mL	0.5371 mL	1.0742 mL	2.6855 mL
	30 mM	0.0895 mL	0.4476 mL	0.8952 mL	2.2379 mL

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Isosinensetin

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