MC-ND-18

MC-ND-18 (100-200 nM; 24 h) potently inhibits IL-1β secretion in PMA-differentiated THP-1 cells, with an inhibition rate of 73.6% at 200 nM and 45.8% at 100 nM^[1].
MC-ND-18 (100-500 nM; 24 h) induces concentration-dependent degradation of NLRP3 in PMA-differentiated THP-1 cells, with degradation rates of 65% at 500 nM, 49% at 200 nM, and 13% at 100 nM^[1].
MC-ND-18 (50 nM-1 μM; 24 h) induces NLRP3 degradation in PMA-differentiated THP-1 cells, with a DC₅₀ of 125.5 nM, and the maximum degradation rate reaches up to 70% at 500 nM^[1].
MC-ND-18 (50 nM-5 μM; 24 h) exhibits low cytotoxicity in PMA-differentiated THP-1 cells, and no significant reduction in relative cell viability is observed after incubation at concentrations ranging from 50 nM to 5 μM for 24 h^[1].
MC-ND-18 (19.5 nM-5 μM) binds to purified NLRP3 protein lacking the LRR domain and LC3B protein, with K_d values of 0.929 μM and 3.548 μM, respectively, indicating that it exhibits concentration-dependent affinity for both targets^[1].
MC-ND-18 (24 h) does not degrade NLRP3 in PMA-differentiated THP-1 cells via the proteasomal pathway, as combined treatment with the proteasome inhibitor MG132 does not attenuate its degrading activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.