country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

Cat. No.: HY-18198

Copy Link Print Information

BRN-103

BRN-103 is a nicotinamide derivative and an inhibitor of the VEGF/VEGFR2 angiogenesis signaling pathway. BRN-103 concentration-dependently inhibits VEGF-induced migration, proliferation and capillary-like tube formation of HUVECs, and suppresses microvascular sprouting of ex vivo aortic rings. BRN-103 inhibits VEGF-induced phosphorylation of VEGFR2 Tyr1175, as well as the downstream phosphorylation of AKT Ser473 and eNOS Ser1172, while exerting a weak inhibitory effect on ERK phosphorylation. BRN-103 can be used in studies related to VEGF/VEGFR2 signaling and angiogenesis.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No.: 1346265-80-2
화학식: C₂₄H₂₅ClN₄O
분자량:420.94

보관:

Please store the product under the recommended conditions in the Certificate of Analysis.

BRN-103 Chemical Structure

CAS No.: 1346265-80-2

Amount: USD 0.00

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- In Vitro
Chemical Information
순도&문서
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

IC₅₀ & Target

^[1]

VEGFR2

All VEGFR Isoforms :

In Vitro

BRN-103 (0.1-10 μM; 24 h) inhibits VEGF-induced migration of human umbilical vein endothelial cells in Transwell assays in a dose-dependent manner^[1].
BRN-103 (0.1-1 μM; 1 h pre-incubation, 24 h incubation with VEGF) inhibits VEGF-induced scratch wound healing migration of human umbilical vein endothelial cells in a dose-dependent manner^[1].
BRN-103 (0.1-1 μM) inhibits VEGF-induced proliferation of human umbilical vein endothelial cells (HUVECs) in a dose-dependent manner^[1].
BRN-103 (0.1-1 μM) dose-dependently inhibits VEGF-induced capillary-like tube formation in human umbilical vein endothelial cells^[1].
BRN-103 (0.1-1 μM; 1 h pre-incubation, 5 min incubation with VEGFR2) dose-dependently inhibits VEGF-induced phosphorylation of VEGFR2 (Tyr-1175) in human umbilical vein endothelial cells^[1].
BRN-103 (0.1-1 μM; 1 h pre-incubation, followed by 5 min, 30 min, or 1 h of incubation with VEGF) dose-dependently inhibits VEGF-induced phosphorylation of AKT (Ser-473) and eNOS (Ser-1172), and slightly inhibits phosphorylation of ERK (Thr-202/Tyr-204) in human umbilical vein endothelial cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

human umbilical vein endothelial cells (HUVECs)
Concentration:

0.1 μM, 0.5 μM, 1 μM
Incubation Time:

1 h pre-incubation; 5 min incubation with VEGF
Result:

Dose-dependently suppressed VEGF-induced phosphorylation of VEGFR2 at Tyr-1175 in HUVECs, with greater inhibition observed at higher concentrations.

Western Blot Analysis^[1]

Cell Line:

human umbilical vein endothelial cells (HUVECs)
Concentration:

0.1 μM, 0.5 μM, 1 μM
Incubation Time:

1 h pre-incubation; 5 min (p-ERK), 30 min (p-AKT), 1 h (p-eNOS) incubation with VEGF
Result:

Significantly and concentration-dependently suppressed VEGF-triggered phosphorylations of AKT (Ser-473) and eNOS (Ser-1172) in HUVECs.
Mildly inhibited VEGF-induced phosphorylation of ERK (Thr-202/Tyr-204) in HUVECs.

Chemical Information

CAS No. 1346265-80-2
분자량 420.94
화학식 C₂₄H₂₅ClN₄O
SMILES

O=C(NC=1C=CC=C(Cl)C1)C2=CC=CN=C2NC3CCN(CC=4C=CC=CC4)CC3
선적

Room temperature in continental US; may vary elsewhere.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

순도&문서

Handling Instructions (2659 KB)

References

[1]. Choi HE, et al. BRN-103, a novel nicotinamide derivative, inhibits VEGF-induced angiogenesis and proliferation in human umbilical vein endothelial cells. Bioorganic & medicinal chemistry letters. 2011 Nov 01;21(21):6236-41.

Calculators

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

견적 받기 해외재고보유

Other size

견적 받기

Amount: USD 0.00

Biological Activity
- 제품 설명
- IC₅₀ & Target
- In Vitro
Chemical Information
순도&문서
References

상품명:
Cat. No.:
수량:
MCE Japan Authorized Agent:

상품명:
CAS No.:
Species:
Purity:
수량:

BRN-103

All VEGFR Isoforms

Biological Activity

제품 설명

IC50 & Target

In Vitro

Chemical Information

순도&문서

References

Calculators

Help & FAQs

BRN-103 Related Classifications

HY-18198 Related Classifications

Bioz

Keywords

Your Recently Viewed Products

IC₅₀ & Target