Development of a long noncoding RNA BC032469-dependent gold nanoparticle molecular beacon for the detection of gastric cancer cells

  • Nanomedicine (Lond). 2021 Oct;16(25):2255-2267. doi: 10.2217/nnm-2021-0249.
Zhuo Yang  1 ,  En Liu  1 ,  Su Min Wang  1 ,  Yu Feng Xiao  1 ,  Shuo Zeng  1 ,  Shi Ming Yang  1 ,  Xiao Yan Zhao  1 ,  Yu Huang  1
Affiliations
  • 1. Department of Gastroenterology, Xinqiao Hospital, Army Medical University, No. 83, Xinqiao Street, Shapingba District, Chongqing, 400037, China.
PMID: 34569291 DOI: 10.2217/nnm-2021-0249
Abstract

Aim: Long noncoding RNA (lncRNA) BC032469-dependent gold nanoparticle molecular beacons (AuNP-MB) were constructed for the detection of gastric Cancer cells. Materials & methods: The AuNP-MBs were prepared according to well-established procedures based on the Au-S interaction between the gold lattice and thiol functionalized oligonucleotides. More importantly, the stability and targeting ability of AuNP-MB were verified by a series of in vitro and in vivo experiments. Results: The lncRNA-dependent probes were successfully utilized for AuNP-MB-based intracellular imaging, with fluorescence effectively emitted in GC cells, but not in normal cells. Notably, such fluorescent emission was positively correlated with lncRNA BC032469 expression. Conclusion: The authors developed an effective fluorescent imaging probe for the recognition of gastric Cancer cells.

Keywords
diagnosis; fluorescence; gastric cancer; gold nanoparticle (AuNP); long noncoding RNAs (lncRNA); molecular beacons.
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